菌体携带孤儿抗毒素类酶,以中和DarTG1毒素-抗毒素防御系统
Anna Johannesman1, Nico A Carlson1, Michele LeRoux1
1Department of Molecular Microbiology, Washington University in Saint Louis School of Medicine, Saint Louis, MO 63110, USA.
bioRxiv : the preprint server for biology
|July 19, 2024
概括
菌体已经进化出一种新的抗DarT因子 (AdfN),以克服细菌的防御. 这种孤儿蛋白质是NADAR超级家族的一部分,可以对抗DarTG1毒素-抗毒素系统,从而使菌体复制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 细菌使用众多的反菌防御系统来限制菌体的复制.
- 反过来,菌体拥有反防御机制来克服这些细菌系统.
- 许多菌体反防御的进化起源在很大程度上是未知的.
研究的目的:
- 识别和描述新型菌体对抗细菌毒素-抗毒素系统的防御机制.
- 阐明T4类菌体中孤儿抗毒素元素的分子功能和进化起源.
主要方法:
- 生物信息分析用于识别孤儿菌素蛋白.
- 生物化学测试以确定酶活性 (ADP-ribosylglycohydrolase).
- 在细菌防御系统的存在下,菌体感染测定.
主要成果:
- 在T4类菌体中发现一个孤儿抗DarT因子 (AdfN).
- AdfN作为ADP-ribosylglycohydrolase的功能,可以从菌体DNA中去除ADP-ribose的修饰.
- 在使用DarTG1毒素-抗毒素系统的细菌中,AdfN可以实现菌体的复制.
- 其他菌体中的相关NADAR超级家族蛋白也表现出抗DarTG1活性.
结论:
- AdfN代表了一种针对细菌DarTG1系统的新型菌体反防御.
- NADAR超级家族通过ADP-ribosylation在细菌菌相互作用中发挥着重要作用.
- 这项研究揭示了以前未被描述的NADAR蛋白的子集的功能.
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