孕产妇的肥胖和后代的神经发育与缺氧基因表达有关,即人类胎盘
bioRxiv : the preprint server for biology
|July 19, 2024
概括
怀孕期间母亲的肥胖可能会影响后代的神经发育. 胎盘细胞的缺氧称为外性热囊细胞 (EVTs) 与这种风险有关,这表明它在发育编程中起着关键作用.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 孕产妇肥胖 (MO) 影响了美国三分之一的怀孕.
- MO与后代的不良神经发育和代谢结果有关.
- 胎盘对于胎儿发育和规划长期健康至关重要.
研究的目的:
- 为了比较胎盘基因表达在孕产妇肥胖与瘦身条件.
- 确定将母亲肥胖与后代神经发育风险联系在一起的机制.
- 调查胎盘缺氧在这个过程中的作用.
主要方法:
- 从MO和瘦胎怀孕中对人类胎盘组织的单核RNA测序.
- 对母体面向侧面和胎儿面向侧面的胎盘转录组的分析.
- 在一个独立的队列 (Gen3G) 中验证,使用大量的胎盘RNA-seq和培养的EVTs.
主要成果:
- 孕产妇的肥胖在多种胎盘细胞类型中的低氧反应基因上调.
- 胎盘缺氧基因表达与后代神经发育指标相关.
- 与神经发育受损相关的基因在异位囊细胞 (EVTs) 中表达高.
- 在培养EVT中的低氧暴露增加了神经发育障碍基因得分.
结论:
- 外性热囊细胞 (EVT) 的缺氧可能会调解母亲肥胖和后代神经发育风险之间的关联.
- 这种胎盘缺氧可能是发育规划中的关键机制.
- 向胎盘缺氧可能为减轻MO相关风险提供治疗途径.
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