在接种流感病毒疫苗或感染后对HA的多克隆IgG反应的结构映射
André Nicolás León1, Alesandra J Rodriguez1, Sara T Richey1
1Department of Integrative Structural and Computational Biology, The Scripps Research Institute, San Diego, CA.
bioRxiv : the preprint server for biology
|July 19, 2024
概括
季节性流感疫苗和感染引发了针对保留流感血素区域的抗体. 接种疫苗的个体比感染者表现出更广泛的表位反应,为通用流感疫苗设计提供了信息.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 结构生物学 结构生物学
背景情况:
- 了解对流感病毒感染和疫苗接种的免疫反应对于提高疫苗疗效至关重要.
- 季节性流感疫苗的目的是引起对流行的流感菌株的保护性免疫力.
研究的目的:
- 在季节性流感疫苗接种或流感A病毒感染后,对血凝素 (HA) 的幽默性IgG抗体反应进行结构性表征.
- 为了确定普遍流感疫苗开发的保存目标表位.
主要方法:
- 使用负染色电子显微镜多克隆表皮图映射 (nsEMPEM).
- 在疫苗接种或感染后对人类患者IgG抗体反应的分析.
主要成果:
- 接种疫苗和感染的个体都产生了针对H1和H3亚型的保存HA区域 (茎,) 的抗体.
- H1反应主要针对茎,而H3反应主要针对头部表位.
- 接种疫苗的捐赠者,平均而言,与感染患者相比,对表位组的多样性更大.
结论:
- 针对流感疫苗接种和感染的抗体反应针对保护区域,包括潜在的通用疫苗目标.
- 接种疫苗可能会引起比自然感染更广泛的表皮图识别.
- 数据为评估多克隆抗体反应和指导未来疫苗设计提供了基线.
相关概念视频
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
00:42Cross-reactivity
Overview
01:51Vaccinations
Overview
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:25Antibody Structure and Classes
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
01:19Hypersensitivity Reactions: Immune-Complex Reactions
Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum sickness, a systemic...