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像Vestigial 4一样,它调节了古典棕色脂肪组织的脂肪生成

Pingzhu Zhou¹, Chase W Kessinger², Fei Gu¹

¹Boston Children's Hospital, 300 Longwood Ave, Boston, MA, 02115.

bioRxiv : the preprint server for biology

|July 19, 2024

概括

像4 (VGLL4) 样的体对棕色脂肪组织 (BAT) 发育至关重要. 破坏VGLL4会影响BAT增长,而其过度表达会增加BAT体积和耐寒性.

科学领域:

细胞生物学细胞生物学
发育生物学是发展生物学.
代谢过程中的代谢.

背景情况:

棕色脂肪组织 (BAT) 是哺乳动物非热发生的关键.
控制BAT生长和脂肪生成的分子机制在很大程度上是未知的.
河马-YAP通路调节器官大小;像4 (VGLL4) 这样的垂体调节这个通路.

研究的目的:

研究VGLL4在调节棕色脂肪组织发育中的作用.
了解VGLL4影响BAT脂肪生成的分子机制.

主要方法:

一个常规的Vgll4突变小鼠线与破坏的Tondu (TDU) 域的生成.
组织学分析和磁共振成像 (MRI) 来评估BAT形态和脂肪生成.
在棕色脂肪细胞中,由腺相关病毒 (AAV) 介导的VGLL4过度表达.

主要成果:

Vgll4 突变小鼠表现出围产死亡率和减少的双肩间BAT.
在Vgll4突变的BAT中,脂肪发生障碍通过组织学和MRI得到证实.
成年雄性小鼠中的VGLL4过度表达增加了BAT体积,并提供了对寒冷应激的保护.

结论:

在调节棕色脂肪组织发育方面,VGLL4起着至关重要的,以前未知的作用.
在BAT中,VGLL4/TEAD1复合体直接影响肌原性和脂肪原性基因表达.
VGLL4被确定为一个关键的脂肪生成因子,对于经典的BAT开发至关重要.

关键词:

在CEBPβββ中.河马-YAP通道的路径.我们的 TEAD1 团队.像4个这样的遗迹.脂质生成 (adipogenesis) 是一种经典的棕色脂肪组织.发展发展发展发展发展.

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