相关实验视频

Updated: Jun 20, 2025

The Soft Agar Colony Formation Assay

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

LncRNA ZFPM2-AS1通过与CDC42结合并抑制STAT1激活来促进植物瘤的进展

Shishi He^1,2, Guowei Huang³, Rong Lei^1,2

¹Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.

Acta pharmaceutica Sinica. B

|July 19, 2024

概括

长非编码RNAZFPM2-AS1在恶性乳腺类瘤 (PT) 中过度表达,导致瘤进展. 向ZFPM2-AS1抑制PT生长,这表明它是乳腺PT的预后标志物和治疗标.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

乳腺囊瘤 (PT) 是一种罕见的有恶性潜力的纤维细胞瘤.
长非编码RNAs (lncRNAs) 在乳腺PT病变发生中的作用在很大程度上是未知的.

研究的目的:

通过微阵列分析,研究lncRNAs在乳腺PT中的作用.
确定与恶性PT相关的特定lncRNA,并探索它们的功能机制和治疗潜力.

主要方法:

微阵列分析以确定PT中的差异表达的lncRNA.
在体外实验中评估ZFPM2-AS1对PT细胞增殖,迁移和入侵的功能影响.
在体内研究使用患者衍生异种移植 (PDX) 模型与siRNA输送以准ZFPM2-AS1.1.
涉及RNA-蛋白结合试验和途径分析的机制研究.

主要成果:

lncRNA ZFPM2-AS1在恶性PT中显著上调,与高瘤等级和不良预后相关.
在体外,ZFPM2-AS1的过度表达促进了恶性PT细胞的增殖,迁移和入侵.
在PDX模型中用siRNA准ZFPM2-AS1有效地抑制了体内瘤的进展.
ZFPM2-AS1竞争性地结合CDC42,抑制ACK1和STAT1的激活,导致TNFRSF19的转录.

结论:

ZFPM2-AS1在乳腺PT的发展和进展中发挥着关键作用.
在乳腺PT患者中,ZFPM2-AS1作为潜在的预后生物标志物.
ZFPM2-AS1代表了乳腺PT治疗的有前途的治疗标.

关键词:

已激活的cdc42激酶1 细胞分裂周期 42 细胞分裂周期 42 这是一个LncRNARNA.纳米粒子是纳米粒子.菲洛德 (Phyllodes) 的瘤是一种瘤.信号传感器和转录激活器 1 1 1 作为TNF受体超级家族成员19,在 ZFPM2-AS1 中,

更多相关视频

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.