生物流体中微量元素的分析性能规范来自六个国家的联合外部质量评估方案,长达10年
Josiane Arnaud1, Cas Weykamp2, Ross Wenzel3
1Member of French Society for Clinical Biology (SFBC), and French Speaking Society for Trace Elements, Vitamins and Biofactors (SETViB), Paris, France.
Clinical chemistry and laboratory medicine
|July 19, 2024
概括
本研究介绍了分析性能规范 (APS),使用在微量元素分析中进行实验室熟练测试的特征功能. 该方法很有用,但对某些元素和矩阵有局限性.
科学领域:
- 临床化学 临床化学
- 环境医学 环境医学
- 实验室科学 实验室科学
背景情况:
- 外部质量评估 (EQA) 方案对于评估实验室能力至关重要.
- 需要分析性能规范 (APS) 来设定实验室测试的性能目标.
- 生物样本中的微量元素分析对质量控制提出了独特的挑战.
研究的目的:
- 定义用于评估实验室能力的分析性能规范 (APS).
- 将普森的特征函数应用于EQA数据来导出APS.
- 评估这种方法对生物矩阵中微量元素的有用性和局限性.
主要方法:
- 利用了来自职业和环境实验室医学 (OELM) EQA计划的可靠数据.
- 应用了普森的特征函数,基于不同度的平均CV和SD.
- 定义APS是从特征函数模型中得出的1.65标准偏差.
主要成果:
- 成功计算了血清,全血和尿液矩阵中的18个元素的APS.
- 对于接近检测极限或具有高可变性的元素,确定了限制.
- 发现了与表现出与特征函数相对不平衡的强硬标准偏差的元素的问题.
结论:
- 普森的特征函数提供了一个可行的方法来导出APS当生物变异数据是不可用的.
- 该方法的适用性对于某些元素是有限的,需要仔细考虑矩阵和元素特征.
- 来自OELM的EQA数据强调了对特征函数方法的元素特定验证的需要.
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