Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Artificial repression looping control by regulated autoinhibition of transcription activator-like effector dimer proteins.

Nucleic acids research·2026
Same author

Iron-addicted colorectal cancers exploit heme-complex II axis to resist oxidative cell death.

Cell metabolism·2026
Same author

Unbiased CRISPR synthetic lethal screening for genetic vulnerabilities in a succinate dehydrogenase-loss model of paraganglioma.

iScience·2026
Same author

Defining the DNA Binding Specificity of GRHL2.

bioRxiv : the preprint server for biology·2026
Same author

Cell-SELEX identifies a DNA aptamer for highly selective in vivo siRNA delivery in cholangiocarcinoma.

JHEP reports : innovation in hepatology·2026
Same author

<i>In Vivo</i> Selection of anti-glioblastoma DNA aptamer-drug conjugates in an orthotopic patient-derived xenograft model.

bioRxiv : the preprint server for biology·2026

相关实验视频

Updated: Jun 20, 2025

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

17.8K

活体细菌中DNA/蛋白质复合体的高分辨率表征

Nicole A Becker1, Justin P Peters1,2, L James Maher3

  • 1Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Rochester, MN, USA.

Methods in molecular biology (Clifton, N.J.)
|July 19, 2024
PubMed
概括
此摘要是机器生成的。

DNA循环对于基因调节至关重要. 新的方法,ChIP-exo和ChEC,在体内绘制了DNA-蛋白相互作用图,证实了大肠杆菌的结构性蛋白质结合.

关键词:
建筑蛋白质是一种建筑蛋白质.染色体内源性裂变 (ChEC) 是一种染色体免疫沉 (ChIP) 是一种高分辨率地图绘制.拉克镇压循环是一个循环.结合介导的PCR (LM-PCR) 是一种菌体兰巴达外核酶是一种菌体兰巴达外核酶.聚合酶连锁反应 (PCR) 是一种南方的斑点 南方的斑点

更多相关视频

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.0K
Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K

相关实验视频

Last Updated: Jun 20, 2025

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

17.8K
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM

Published on: September 29, 2014

20.0K
Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

11.8K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 微生物学 微生物学

背景情况:

  • DNA循环是一个基本的生物过程,涉及 prokaryotes 和 eukaryotes 的基因调节.
  • 大肠杆菌乳糖 (lac) 操作子作为研究基因调节机制的模型系统,包括DNA循环.
  • 了解细菌核体内的DNA-蛋白相互作用对于破译基因表达控制至关重要.

研究的目的:

  • 开发和介绍两种新的,互补的方法,用于高分辨率的DNA/蛋白质结合的体内检测.
  • 通过应用这些方法来验证这些方法,以研究特征很好的大肠杆菌 lac operon 中的 DNA 循环.
  • 提供一个详细的地图的建筑蛋白质结合部位参与DNA抑制循环.

主要方法:

  • 染色体免疫沉与菌体λ外核酶消化 (ChIP-exo) 结合,用于体内DNA/蛋白质映射.
  • 染色体内源性裂变 (ChEC) 与结介导的聚合酶链反应 (LM-PCR) 和南方斑点分析相结合.
  • 这些技术应用于大肠杆菌以分析细菌核体内的蛋白质结合.

主要成果:

  • 成功的高分辨率,在体内绘制的DNA-蛋白相互作用在细菌核体内.
  • 在E. coli中,Lac抑制剂介导的DNA抑制循环中直接结合结构蛋白的证明.
  • 验证ChIP-exo和ChEC作为研究DNA循环和蛋白质结合的强大工具.

结论:

  • ChIP-exo和ChEC是有效的补充方法,用于高分辨率的DNA-蛋白相互作用的体内分析.
  • 这些方法直接证实了 prokaryotic 基因调节中的结构性蛋白质结合,以大肠杆菌 lac operon 为例.
  • 这项研究提升了我们研究活体细菌细胞中DNA和蛋白质复合物的结构动态的能力.