相关实验视频

Updated: Jun 20, 2025

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

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遗传方法研究转录与大肠杆菌中核蛋白相关蛋白之间的相互作用

Aathmaja Anandhi Rangarajan1,2, Cihan Yilmaz1,3, Karin Schnetz4

  • 1Institute for Genetics, University of Cologne, Cologne, Germany.

Methods in molecular biology (Clifton, N.J.)
|July 19, 2024
PubMed
概括

研究人员开发了新的遗传工具来研究细菌转录如何影响Escherichia coli中的H-NS等核相关蛋白 (NAP). 这些方法有助于理解DNA超级卷,转录和NAP结合之间的复杂相互作用.

关键词:
附着部位的位置染色体的整合.与核蛋白相关的蛋白质重组组合是一种重组.转录条例 转录条例 转录条例 转录条例转导 转导是指转导的过程.

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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

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Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization10:01

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Published on: July 30, 2020

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RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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