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用响应表面方法优化解热保护剂对复合人体酸性纤维细胞生长因子的优化

Qiwen Huang¹, Jing Hu¹, Dongjie Mei¹

¹College of Life Science, Yangtze University, Jingzhou, PR China.

Preparative biochemistry & biotechnology

|July 19, 2024

概括

这项研究优化了对复合人体酸性纤维细胞生长因子 (rh-aFGF) 的冷干燥配方,以提高稳定性和生物活性. 最好的配方确保了药物开发的最佳冷干燥特性和细胞增殖.

科学领域:

生物技术是生物技术.
制药科学制药科学
蛋白质稳定蛋白质稳定

背景情况:

重组人体酸性纤维细胞生长因子 (rh-aFGF) 是一种有价值的生物产品.
rh-aFGF表现出不稳定性和随着时间的推移失去生物活动的倾向.

研究的目的:

优化rh-aFGF的冷干燥配方,以确保长期稳定性和保持生物活性.
使用优化的辅助剂评估rh-aFGF的冷干燥特性和细胞增殖率.

主要方法:

使用响应表面方法来优化辅助剂度.
使用冷干燥表征和甲基亚二四化物 (MTT) 试验来评估稳定性和活性.
回归实验验证实了最佳配方.

主要成果:

三糖,甘氨酸和牛血清白蛋白 (BSA) 显著影响了解的rh-aFGF特征和细胞增殖.
最佳配方包括5.7%的三糖,2.04%的甘氨酸和1.98%的BSA.
经过验证的配方实现了冷干燥的可塑性指数为23.35,细胞增殖率为43.59%.

结论:

成功确定了rh-aFGF的稳定冷干燥配方.
这种配方保持了冷干燥完整性和生物活性.
这些发现为开发基于rh-aFGF的药物提供了基础.

关键词:

在冷干燥过程中进行冷干燥.解热保护剂是什么重组的人类酸性纤维细胞生长因子.表面响应的表面响应.

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