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Updated: Aug 10, 2026

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells MSCs and Endothelial Colony Forming Progenitor Cells ECFCs
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隔离和识别来自人类带介质干细胞的小细胞外囊的协议

Yanxia Chen1, Hui Qian2, Michael Mak3

  • 1Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China.

STAR protocols
|July 19, 2024
PubMed
概括

这项研究详细介绍了从人类带介质干细胞 (hucMSCs) 中分离小细胞外囊泡 (sEVs) 的协议. 该方法可确保对研究应用中的SEV进行可靠的表征.

关键词:
生物技术和生物工程细胞生物学 细胞生物学细胞隔离细胞的隔离.

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相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.