在巨细胞中,CircARCN1通过调节HuR介导的USP31mRNA来加剧动脉样硬化
Zhicheng Pan1, Jialan Lv1, Liding Zhao1
1Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Cardiovascular research
|July 19, 2024
概括
循环RNA ARCN1 (circARCN1) 在冠状动脉疾病 (CAD) 患者中升高,通过调节炎症和巨细胞中的NF-κB激活来促进动脉样硬化. 这表明circARCN1是动脉样硬化病变形成的潜在因素.
科学领域:
- 分子生物学分子生物学
- 心血管研究研究心血管研究
- 生物化学 生物化学
背景情况:
- 循环RNAs (circRNAs) 是生物过程的关键调节者.
- 循环RNAs在动脉样硬化中的作用是冠状动脉疾病 (CAD) 的主要原因,需要进一步阐明.
- 在CAD患者中研究circRNA可以揭示对疾病发病因子的新见解.
研究的目的:
- 研究circRNAs在动脉样硬化发展中的作用.
- 探索circRNAs作为CAD生物标志物的潜力.
- 阐明circRNA参与CAD的潜在机制.
主要方法:
- 从CAD患者和对照者的血细胞中转录组查和量化circRNAs.
- 在体外和体内研究使用增益和丧失功能的方法.
- 涉及HuR,USP31mRNA,NF-κB信号传递和ApoE-/-小鼠模型的机制研究.
主要成果:
- 在CAD患者的外周血液单核细胞和动脉斑块中发现circARCN1表达升高.
- circARCN1水平与稳定性心痛 (SA) 和急性冠状动脉综合征 (ACS) 的风险相关.
- circARCN1调节HuR介导的USP31mRNA稳定性,减轻NF-κB激活和减少巨细胞的炎症,从而影响动脉样硬化病变的发展.
结论:
- 巨细胞表达的circARCN1在动脉样硬化发展中起着重要作用.
- circARCN1通过调节HuR-USP31 mRNA稳定性和NF-κB信号传递来影响动脉样损伤的形成.
- circARCN1代表了潜在的治疗标和在CAD中形成动脉样硬化病变的生物标志物.
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