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Updated: Jun 20, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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在人类血清中使用单颗粒ICP-MS和金纳米粒子作为标签的miRNA量化无放大策略

Sara González Morales1,2, Carlos López-Portugués1,2, Manuel Fernández-Sanjurjo3,2

  • 1Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.

Analytical chemistry
|July 19, 2024
PubMed
概括

一种新的方法在没有放大的情况下分析血中的microRNAs (miRNAs),将结果与单颗粒ICP-MS相关联. 这种敏感的测试基于miRNA生物标志物来区分久坐和运动的个体.

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Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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