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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jun 20, 2025

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
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Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

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自动闪的蒂奥夫拉T用于超分辨率成像.

Qiqi Yang1, Elnaz Hosseini1, Peigen Yao1

  • 1Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.

The journal of physical chemistry letters
|July 19, 2024
PubMed
概括
此摘要是机器生成的。

提奥夫拉T (ThT) 染料对单分子超分辨率成像表现出固有的随机闪,这与之前的看法相反. 这种特性使得无需专门的缓冲器,可以可视化粉样纤维.

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科学领域:

  • 生物物理学的生物物理.
  • 化学生物学是化学生物学.
  • 显微镜的使用方法

背景情况:

  • 提奥夫拉T (ThT) 是一种用于检测粉样和类纳米纤维的标准染料.
  • 一般认为ThT在结合聚合蛋白时具有稳定的光.
  • 单分子定位显微镜 (SMLM) 需要光交换/闪特性来进行超分辨率成像.

研究的目的:

  • 在单个分子水平上研究ThT的内在光交换/闪特性.
  • 为了确定ThT是否可以在没有专门的闪缓冲器的情况下用于SMLM.
  • 在各种实验条件下描述ThT的闪行为.

主要方法:

  • 单分子光谱学分析ThT光.
  • 在超低度下研究ThT闪特性 (光子数量,持续时间,开关比).
  • 在空气和PBS缓冲器中对THT标记的α-synuclein纤维的SMLM成像.

主要成果:

  • 在稳定的约束条件下,ThT显示了内在的随机闪,这与之前的假设相矛盾.
  • 在单个分子水平上量化了ThT的闪特征.
  • 使用ThT.成功地实现了α-synuclein纤维的SMLM成像.

结论:

  • ThT具有固有的光交换能力,适合SMLM.
  • 基于ThT的粉样结构的SMLM不需要专门的闪缓冲器.
  • 这一发现扩大了ThT在蛋白质聚合物的超分辨率显微镜中的实用性.