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hPMSCs通过促进GSH合成来预防由移植与宿主疾病引起的红细胞功能障碍

Yanlian Xiong¹, Feifei Wang², Huanmei Mu³

¹Department of Histology and Embryology, School of Basic Medicine, Binzhou Medical University, Yantai, PR China.

International immunopharmacology

|July 19, 2024

概括

人类胎盘衍生中细胞 (hPMSCs) 通过增加谷氨 (GSH) 生产来提高红细胞的抗氧化能力,从而改善异构干细胞移植后的移植与宿主疾病 (GVHD).

科学领域:

血液学血液学血液学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

异质造血干细胞移植 (allo-HSCT) 接受者经历氧化应激增加,导致移植与宿主疾病 (GVHD).
众所周知,介酶体 stromal 细胞 (MSC) 能够调节 T 细胞并改善 GVHD.
在GVHD中MSCs在调节红细胞抗氧化物代谢中的作用仍然未被探索.

研究的目的:

研究人类胎盘衍生MSCs (hPMSCs) 对红细胞抗氧化物代谢在GVHD的背景下的影响.
为了确定hPMSC治疗是否可以通过改善红细胞抗氧化能力来减少GVHD.

主要方法:

使用了体外超氧化物生产和体外GVHD小鼠模型.
分析了红细胞超结构,可变形性和膜蛋白表达 (带3,β-光谱).
评估了氧化损伤,抗氧化酶活性和谷氨 (GSH) 水平.

主要成果:

GVHD诱导了红细胞形态和可变形缺陷,带3和β-谱表达减少.
来自GVHD患者和模型小鼠的红细胞显示氧化应激增加和抗氧化能力降低.
hPMSC疗法逆转了GVHD诱导的红细胞氧化还原失衡,主要是通过上调葡萄糖代谢来增强GSH合成和循环.

结论:

hPMSCs通过增加GSH生产来增强红细胞的抗氧化能力.
这种机制有助于改善alo-HSCT接受者的GVHD.
准红细胞抗氧化代谢是GVHD的潜在治疗策略.

关键词:

红细胞是红细胞.这是GSHGSH.在GVHD中,GVHD是指GVHD.氧化应激是一种氧化应激.hPMSCs的使用情况.

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