相关实验视频

Updated: Jul 22, 2026

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

通过基于化学衍生物的LC-MS/MS,对梅瓦诺酸路径中间体的敏感量化和相对新型类型的预测

Meiyu Gao¹, Jiarui Sun¹, Qinwen Xiao¹

¹Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 210009, PR China.

Journal of chromatography. A

|July 19, 2024

概括

这项研究开发了一种新的LC-MS/MS方法,用于分析甲酸途径中间体,识别新代谢物并改善疾病研究. 该方法增强了对各种健康和疾病状况中的美瓦酸途径的理解.

科学领域:

代谢学代谢学代谢学
生物化学生物化学
分析化学分析化学

背景情况:

梅瓦酸盐 (MVA) 途径在癌症和骨质疏松症等疾病中至关重要.
分析MVA途径中间体是具有挑战性的,因为它们的属性 (极性,低度,化,MS反应差).

研究的目的:

建立一种敏感的LC-MS/MS方法,用于同时分析所有MVA中间体.
为了确定新的MVA途径代谢物,并探索它们的组织分布.
为了研究MVA中介差异在耐思的非小细胞肺癌 (NSCLC) 细胞中.

主要方法:

开发了一种液体染色学-双重质谱法 (LC-MS/MS) 方法.
使用N2,N2,N4,N4-四甲基-6-(4-(piperazin-1-ylsulfonyl) ) -1,3,5-三-2,4-二胺 (Tmt-PP) 标签进行衍生.
使用质谱计算来绝对量化异构体 (IPP和DMAPP).
应用知识驱动的预测策略,用于新型代谢物识别.

主要成果:

化学衍生改善了分析物保留,峰值形状和检测灵敏度.
成功量化了异构体异烯酸盐 (IPP) 和二甲基烯酸盐 (DMAPP) 的数量.
首次确定了几种新的MVA中间体类型 (IMP/DMAMP,GMP,MTP,ITP/DMATP).
研究了这些新发现的代谢物的组织分布.
观察到MVA中间体的差异在耐西斯普拉丁NSCLC细胞中.

结论:

为MVA中间体开发了一种敏感和全面的量化方法.
该研究确定了新的MVA途径代谢物,扩大了已知的代谢格局.
这项工作提高了对MVA途径在健康和疾病中的作用的理解,特别是在NSCLC中.
新发现的代谢物需要进一步研究它们的生物合成和功能.

关键词:

化学衍生物化化学衍生物化药物耐药性药物耐药性药物耐药性这是LC-MS/MS.质谱计算质谱计算梅瓦诺酸的途径

更多相关视频

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...