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TDRD1阶段分离驱动了线粒体间的水泥组件,以促进piRNA生物发生和生育能力

Jie Gao¹, Jiongjie Jing², Guanyi Shang¹

¹Shanghai Key Laboratory of Maternal Fetal Medicine, Clinical and Translational Research Center, Shanghai First Maternity and Infant Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.

Developmental cell

|July 19, 2024

概括

图多尔域含有1 (TDRD1) 驱动了通过相位分离的线粒体间混凝土 (IMC) 的组装. 这一过程对于生殖细胞中的piRNA生物生成至关重要,其破坏会导致生殖问题.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生殖生物学生殖生物学

背景情况:

介质粒体水泥 (IMC) 是一种胚胎颗粒,对于哺乳动物胚胎细胞中的piRNA生物发生是必不可少的.
在线粒体中IMC组装的机制仍然不太清楚.

研究的目的:

阐明IMC组件背后的分子机制.
为了研究Tudor域含有1 (TDRD1) 在IMC形成和piRNA生物发生中的作用.

主要方法:

使用哺乳动物生殖细胞模型研究了TDRD1在IMC组装中的作用.
分析了TDRD1的相分离特性及其与线粒体的相互作用.
在小鼠模型中研究了TDRD1缺乏症的影响.

主要成果:

TDRD1通过液相分离触发IMC组件,由其卷轴-卷轴和图多尔域驱动.
TDRD1被MILI招募到线粒体中,促进线粒体聚类和IMC形成.
缺少TDRD1会破坏IMC的组合,损害piRNA生物发生,并导致小鼠的精子发生停止.
在IMC组件中,TDRD1介导的相分离在脊椎动物中得到保留.

结论:

TDRD1驱动的相分离是IMC组装和piRNA生物发生的一个关键机制.
这项研究将膜结合的有机体 (线粒体) 与没有膜的有机体 (细菌颗粒) 联系起来.
干扰TDRD1功能对男性生育能力有重大影响.

关键词:

在IMC中,IMC是IMC.千万千万的时间.在TDRD1中,图道尔王朝是什么意思图道尔王朝卷曲的卷曲的卷曲的卷曲细菌颗粒的细菌颗粒.氧化聚合物的一种.阶段分离的阶段分离.这就是为什么piRNARNA.精子的产生是精子的产生.

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