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对防火抗火蛋白FB_MR5的自然变体进行的比较分析表明,有不同的效应因子识别机制

Haseong Kim¹, Jieun Kim², Minseon Kim³

¹Plant Immunity Research Center, Seoul National University, Seoul 08826, Republic of Korea.

Molecules and cells

|July 19, 2024

概括

果病耐药性蛋白FB_MR5 (MrMR5) 中的多态变异改变了其活性和效应因子识别. 在MrMR5的变化影响其与RIN4的相互作用,影响细菌的耐火性.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

由Erwinia amylovora引起的细菌火灾是果园中的一个毁灭性疾病.
核酸结合域和富含白的重复蛋白 (NLR) 赋予植物的抗病能力.
来自Malus × robusta 5的FB_MR5 (MrMR5) 识别了Erwinia amylovora效应剂EaAvrRpt2,并被MdRIN4裂变产品激活.

研究的目的:

研究自然存在的FB_MR5变体中的多态的功能影响.
阐明FB_MR5.5中改变自动活性和效应器识别的分子基础.

主要方法:

来自Malus baccata的FB_MR5 (MrMR5) 和MbMR5的比较分析.
在Nicotiana benthamiana中的短暂表达.
域名交换和突变分析.
调查带有或没有MdRIN的效应器识别4.

主要成果:

与MrMR5不同,MbMR5表现出自动活性,这是由于CC域中单个氨基酸多态性.
具有MbMR5多态的MrMR5识别了AvrRpt2独立于MdRIN4.
多态变异改变了与RIN4的相互作用,影响细胞死亡诱导活性和效应器识别.

结论:

在MR5变体中自然发生的多态性显著影响其抗病功能.
与RIN4的相容性变化是MR5活动和效应器识别变化的关键机制.
这些发现提供了关于NLR介导植物免疫的演变的见解.

关键词:

疾病耐药性基因的疾病耐药性基因燃烧的火灾是因为火灾的火灾.核酸结合域和富含白的重复.病原体效应因子病原体效应因子

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