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工程纳米材料在非常低剂量时对细菌施加次致命的压力:度,光线和介质对细胞膜透性的影响
Shushan Wu1, George Wells1, Kimberly A Gray1
1Department of Civil and Environmental Engineering, Northwestern University, USA.
The Science of the total environment
|July 19, 2024
概括
像二氧化 (n-TiO2) 和银 (n-Ag) 这样的工程纳米材料 (ENM) 在光下变得有毒,增加了细菌外膜的透性. 环境因素和ENM混合物放大了这些次致命效应,影响了生态健康.
科学领域:
- 环境科学 环境科学
- 纳米技术纳米技术
- 毒理学 毒理学 毒理学
背景情况:
- 工程纳米材料 (ENM) 可以破坏细胞功能和基因表达.
- 环境因素对ENM毒性的影响,特别是在亚致命度下,人们对其了解甚微.
研究的目的:
- 评估光,周围介质和ENM混合物对细菌活力和外膜透性的影响.
- 为了研究环境相关的,亚致命度的ENM毒性.
主要方法:
- 在黑暗和模拟的阳光下,大肠杆菌暴露于各种ENM (n-Ag,n-TiO2,n-Al2O3,n-ZnO,n-CuO,n-SiO2).
- 在合成MOPS缓冲器和自然密歇根湖水 (LMW) 中进行测试.
- 评估细胞活力和外膜透性.
主要成果:
- 光激活的n-TiO2和n-Ag的光毒性,在<1 mg/L时增加外膜透性.
- 由于离子强度和组成的差异,LMW中的ENM毒性高于MOPS.
- n-TiO2和n-Ag的混合物显示出超过添加物影响的协同毒性作用.
结论:
- 在自然环境中,ENM及其混合物对细菌产生显著的亚致命压力.
- 这些影响,即使是低剂量,也会导致细胞损伤并影响生态系统的健康.
- 环境因素对ENM的毒性机制产生重大影响.
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