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基于的金属有机框架的分散固相提取与气色谱-质谱相结合,用于确定生物样本中的糖酸盐

Yuyan Hu¹, Boxue Wu¹, Ying-Shu Tang¹

¹Key Laboratory of Precision nutrition and health of Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University Heilongjiang, China.

Analytica chimica acta

|July 19, 2024

概括

新的吸附剂,UiO-66-NH2@SBA-15和ZrO2@SBA-15,可以有效地从生物样本中提取糖酸盐 (SPx). 这种方法提高了检测这些关键的代谢物疾病研究.

科学领域:

分析化学分析化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

糖酸盐 (SPx) 是重要的代谢物,但由于稳定性不佳,丰度低,结构相似,分析具有挑战性.
现有的方法难以在复杂的生物矩阵中有效分离和检测SPx.

研究的目的:

开发新型吸附剂,从生物样本中有效丰富SPx.
建立一种灵敏可靠的方法,用气色谱-质谱法量化SPx.

主要方法:

合成UiO-66-NH2@SBA-15和ZrO2@SBA-15的核心外吸附剂. 这种吸附剂的合成方法是:
使用单变体实验和响应表面方法学优化分散固体相提取条件.
将优化的提取协议与气色谱-质谱相结合,用于SPx检测.

主要成果:

合成的吸附剂显示出对SPx.的高吸附能力和特异性.
优化的方法实现了良好的线性 (5.0-5000.0 ng mL-1),低检测极限 (0.001-1.0 ng mL-1) 和高精度 (RSD <14.7%).
在从血清,唾液和细胞样本中提取SPx时,获得了令人满意的恢复 (89.1-113.8%). 在胃癌患者的SPx分析中,UiO-66-NH2@SBA-15显示了更高的吸附能力 (169.5-196.1毫克g-1).

结论:

UiO-66-NH2@SBA-15是一种有前途的吸附剂,用于从生物样本中提取SPx.
这种方法促进了对SPx代谢变化的研究,并有助于理解疾病的发病性.
开发的技术为临床诊断和代谢研究提供了有价值的工具.

关键词:

生物样本生物样本分散的固体相提取方法金属有机框架的框架.糖类酸盐糖类酸盐 ZrO (((2)) 的使用情况.

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