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一步合成的多尺寸AuAg合金纳米颗粒具有高SERS灵敏度,可直接检测SARS-CoV-2尖端蛋白

Zhenle Qin¹, Yansheng Liu¹, Xiaobo Jia¹

¹School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.

Analytica chimica acta

|July 19, 2024

概括

这项研究提出了一种用于检测SARS-CoV-2的新方法,使用金银合金纳米颗粒作为表面增强拉曼散射 (SERS) 基质. 开发的SERS传感器实现了检测SARS-CoV-2尖端蛋白的高灵敏度和可重复性.

科学领域:

纳米技术纳米技术
生物医学工程生物医学工程
分析化学分析化学

背景情况:

由SARS-CoV-2引起的COVID-19大流行病给全球医疗保健系统带来了压力.
快速准确地检测SARS-CoV-2对疾病控制至关重要.
表面增强拉曼散射 (SERS) 提供了一个有前途的低成本,快速检测方法.

研究的目的:

开发和优化一种基于SERS的传感器,用于检测SARS-CoV-2.
为SERS应用制造和表征金银合金纳米粒子 (AuAgNPs).
评估ACE2-功能化的AuAgNP在检测SARS-CoV-2尖端蛋白中的性能.

主要方法:

一步制造的金银合金纳米粒子 (AuAgNPs) 具有可调的组成和尺寸.
用 ангиотензин转化酶2 (ACE2) 功能化AuAgNP,以创建传感器表面.
在PBS和PSS中使用优化的ACE2/AuAgNPs SERS基质检测SARS-CoV-2尖端蛋白.

主要成果:

优化的ACE2 / Au35Ag65NP在检测SARS-CoV-2尖端蛋白中表现出卓越的性能.
达到了尖端蛋白的低检测极限 (LOD) 10 fg/mL.
在PBS和PSS中表现出出色的可重现性,相对标准偏差 (RSD) 分别为7.7%和7.9%.

结论:

开发的ACE2功能化的AuAgNP作为一个高度敏感和可重复的SERS基质.
这种SERS方法显示了实际和快速检测SARS-CoV-2的巨大潜力.
易于制备的SERS基质有助于推进传染病的诊断工具.

关键词:

在ACE2中,ACE2是ACE2.在AuAgNPs中.在SARS-CoV-2中,S蛋白是S蛋白.表面增强的拉曼散射 (SERS) 是指表面增强的拉曼散射

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