酸对阿克罗巴克特菌sp.的有氧脱的作用. 这是GAD-3菌株
1School of Resources and Environment, Nanchang University, Nanchang 330031, China; Key Laboratory of Poyang Lake Environment and Resource Utilization, Ministry of Education, Nanchang 330031, China.
Journal of bioscience and bioengineering
|July 19, 2024
概括
酸通过增加酸去除率和减少酸积累来增强有氧脱. 然而,它还通过抑制特定的脱基因,增加了氧化排放.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
背景情况:
- 胺酸 (HA) 是天然有机物质的重要组成部分.
- 已知HA会影响无氧脱过程.
- 对于HA对有氧脱物的影响仍然不太了解.
研究的目的:
- 研究酸对Achromobacter sp.的有氧脱的作用. 在GAD-3.0中.
- 阐明HA影响有氧脱的机制.
- 评估废水处理中去除的影响.
主要方法:
- 使用Achromobacter sp.进行有氧脱实验. GAD-3具有不同的HA度.
- 测量酸盐和酸盐去除率.
- 对脱化减少酶活性 (NAR,NIR,NOR) 和N2OR活性进行检测.
- 对关键脱基因 (napA,cnorB,nirS,nosZ) 的基因表达分析.
主要成果:
- 胺酸 (≥5毫克L-1) 显著促进了有氧脱,增加了酸盐去除率 (6.6711.1毫克L-1小时-1).
- HA减少了亚酸盐的积累 (从30.2到20.7毫克L-1).
- HA增强了NAR,NIR和NOR的电子转移和活动,对napA,cnorB和nirS基因表达进行上调.
- HA抑制了nosZ基因表达和N2OR活性,导致N2O排放增加.
结论:
- 胺酸可以提高阿克罗姆巴克特sp.的有氧脱性能. 通过促进酸盐降解通路,增强了GAD-3.
- 通过HA抑制N2O降解对完全去除来说是一个挑战.
- 了解HA的作用对于优化废水处理中的有氧脱至关重要,以改善管理.
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