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基于PacBio HiFi和Hi-C测序的Plagiognathops microlepis的染色体层次的基因组组合

Yidi Wu¹, Hang Sha¹, Xiangzhong Luo¹

¹Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China.

Scientific data

|July 19, 2024

概括

组装了Plagiognathops microlepis的染色体级基因组,提供了对其遗传基础和进化历史的见解. 这种资源有助于对经济上重要的淡水鱼的研究.

科学领域:

基因组学就是基因组学.
水产养殖是水产养殖的一种方式.
进化生物学进化生物学

背景情况:

鱼 (Plagiognathops microlepis) 是一个在中国经济上重要的淡水鱼.
有限的基因组数据阻碍了对其遗传学,系系学和适应性的理解.
这种物种具有中等的经济价值和水产养殖发展潜力.

研究的目的:

为了组装一个高质量的,染色体水平的参考基因组为Plagiognathops microlepis.
提供基因组资源,研究其遗传基础,遗传学状况和适应性进化.
为了支持这个重要的鱼类的保护和繁殖计划.

主要方法:

整合了Pacbio HiFi长读数,Illumina短读数和Hi-C测序数据.
基因组组装和支架到染色体水平.
基因预测和功能注释.

主要成果:

一个1004.34 Mb的基因组与一个38.80 Mb的N50结合组装在一起.
99.59%的序列使用Hi-C数据被定在24个染色体上.
确定了578.91 Mb的重复序列和28337个蛋白质编码基因 (26,929个注释).

结论:

现在可以获得Plagiognathops microlepis的染色体级基因组.
这个资源促进了对鱼类的基因,进化历史和适应策略的研究.
它为了解P. microlepis. 的特征的遗传基础提供了基础.

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