氨酸L-乳化是通过糖解诱导的主要乳化异构体
Di Zhang1,2, Jinjun Gao3,4,5, Zhijun Zhu6
1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China. zhangdi@pku.edu.cn.
Nature chemical biology
|July 19, 2024
概括
Lysine L-lactylation (Kl-la) 是一种关键的蛋白质修饰,与糖解有关. 这项研究将Kl-la与其同体区分开来,确定它是华堡效应的主要响应者.
科学领域:
- 生物化学和分子生物学
- 细胞的新陈代谢
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 氨酸乳化 (Kl-la) 是一种由L-乳酸盐驱动的新型蛋白质后翻译修饰 (PTM).
- 存在三种异构体:Kl-la,N-ε-(carboxyethyl) -lysine (Kce) 和D-lactyl-lysine (Kd-la),在代谢研究中经常引起混.
- 区分这些同位素对于理解它们在细胞过程中的作用至关重要,比如华堡效应.
研究的目的:
- 开发和介绍用于区分 lysine 乳酸化异构体的方法.
- 为了确定参与糖解和华堡效应的初级乳酸化异构体.
- 阐明L-乳糖和它的前体,乳糖共酶A之间的关系.
主要方法:
- 化学衍生物与高性能液态染色学 (HPLC) 结合,用于同位素分离.
- 开发异构体特异性抗体,用于选择性识别乳糖化.
- 对基因素乳化与糖解和糖酶系统活性相关的分析.
主要成果:
- 鉴定出Kl-la是基质子上占主导地位的乳化异构体.
- 与Kl-la和Kce不同的是,Kl-la的水平是通过糖解动态调节的.
- 乳化异构体Kd-la和Kce在不完整的glyoxalase系统条件下被观察到.
- 在乳糖辅酶A水平和Kl-la之间发现了正相关性.
结论:
- 这项研究提供了强大的方法来区分PTM异构体,适用于超出乳化.
- 证实Kl-la是响应糖解和华堡效应的主要异构体.
- 这些发现强调了K在代谢调节中的特殊作用.
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