基因酶功能化的UiO-66框架纳米颗粒对抗多药耐药Candida Auris具有活性
Shaymaa A Ismail1, Bahgat Fayed2, Reda M Abdelhameed3
1Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Institute, National Research Centre, P.O. 12622, 33 El Bohouth Street, Dokki, Giza, Egypt. shaymaaabdallaismail@gmail.com.
BMC microbiology
|July 19, 2024
概括
这项研究增强了基因酶的产生,并将其固定在UIO-66上,显著增强了它对抗耐药酵母 Candida auris (C. auris) 的抗真菌活性. 这种新的方法为对抗C. auris感染提供了一个有希望的新策略.
科学领域:
- 生物技术和生物化学
- 材料科学 材料科学 材料科学
- 抗菌研究 抗菌研究
背景情况:
- 黄菌 (C. auris) 是一种新兴的多药耐药酵母病原体,导致重大疫情.
- 细胞壁成分基对于C. auris抗真菌耐药性至关重要.
- 在纳米结构上的酶固定可以增强酶活性和稳定性.
研究的目的:
- 为了研究在UiO-66上固定化奇丁酶作为抗真菌剂对抗C. auris.的潜力.
- 优化基因酶的产生和描述UiO-66框架.
- 评估固定对奇丁酶活性,稳定性和抗真菌功效的影响.
主要方法:
- 通过使用普莱克特-伯曼和盒子-贝恩肯设计,优化了Talaromyces变异SSW3的基因酶生产.
- 通过SEM,TEM,XRD,FTIR,粒子大小分析和zeta大小测量合成和表征UiO-66纳米材料.
- 在UIO-66上固定了基因酶,并评估了酶动力学,稳定性和对C. auris的抗真菌活性.
主要成果:
- 奇丁酶产量显著提高,达到120.41U/g ds.
- 成功合成和特征UiO-66纳米粒子 (70.42nm) 的研究.
- 在UiO-66上的固定改善了基因酶活性 (Vmax增加2倍,Km减少38倍) 和稳定性,并显著提高了对C. auris的抗真菌功效 (MIC50从5.582减少到0.89U/mL).
结论:
- 在UiO-66上固定基因酶是一种有效的策略,可以增强其抗真菌特性.
- 这种方法为对抗具有挑战性的病原体C. auris.提供了一种新且有效的替代方案.
- 这项研究强调了酶-纳米材料结合物的在抗微生物应用中的潜力.
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