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全基因组DNA甲基化概况及其在秋季军 (Spodoptera frugiperda) 中调节Vip3Aa耐受性的功能

Luming Zou¹, Zhenxing Liu¹, Minghui Jin¹

¹Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, People's Republic of China.

Pest management science

|July 20, 2024

概括

基因甲基化影响秋军虫 (FAW) 对植物性杀虫蛋白 (Vips) 的耐受性. 表观遗传变异,特别是减少基因体甲基化,与FAW对Vip3Aa的耐受性增加相关,提供了新的害虫管理见解.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学
昆虫学昆虫学是一门学科.
虫害管理虫害管理虫害管理

背景情况:

植物性杀虫蛋白 (Vips) 对于害虫控制至关重要,但开发昆虫耐受性,特别是秋季军 (FAW, *Spodoptera frugiperda*),是一个重大挑战.
基因甲基化是环境应激反应中关键的表观遗传机制,其在FAW对Vip3Aa耐受性方面的作用需要调查.

研究的目的:

为秋季军虫 (FAW) 构建一个全基因组的DNA甲基化图.
研究DNA甲基化在调节FAW对Vip3Aa.a的耐受性方面的功能性作用.
探索表观遗传变异与跨世代耐受性之间的关系.

主要方法:

为FAW开发全基因组DNA甲基化图.
对Vip3Aa耐受性和易受性FAW菌株之间的甲基化图的比较分析.
生物测试以评估跨代Vip3Aa耐受性超过十代.
通过RNA干扰 (RNAi) 实验验证基因功能.

主要成果:

前子是FAW基因组中DNA甲基化的首选地点,家政基因显示高甲基化水平.
在FAW人群中观察到Vip3Aa耐受性的跨代增长,这些人群接受了持续查.
基因体甲基化与基因表达正相关. 一个特定的3'5'-循环核酸化酶基因显示甲基化减少,表达减少和Vip3Aa耐受性增加,由RNAi证实.

结论:

这项研究提供了FAW的第一个全基因组DNA甲基化图,阐明了它在Vip3Aa耐受性中的作用.
Vip3Aa查通过DNA甲基化诱导表观遗传变异,有助于耐受性.
了解这些表观遗传机制为管理Lepidoptera中的Vip3Aa耐药害虫提供了新的策略.

关键词:

通过DNA甲基化.它们是花鸟 (Spodoptera frugiperda) 的种类.在Vip3Aa的耐受性.适应适应适应适应

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