长非编码RNA的表达和功能在特有形主义的表达和功能
Yanhong He1,2,3, Jianshuang Li1,2,3,4, Yun Chen1,2,3
1Chinese Centre for Disease Control and Prevention, Centre for Endemic Disease Control, Harbin Medical University, Heilongjiang Province 150081, Harbin City, People's Republic of China.
Molecular neurobiology
|July 20, 2024
概括
这项研究确定了新型长非编码RNA (lncRNA) 和信使RNA (mRNA) 表达变化在特有杂症 (EC) 中. 较高的LINC01220和IDO1水平表明它们在EC病变发生过程中的潜在作用.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 内分泌学 在内分泌学.
背景情况:
- 地方性白痴症 (EC) 是一种严重的缺障碍,遗传贡献不清楚.
- 神经发育障碍和精神缺陷是EC的特征症状.
- 了解EC的遗传基础对于开发有效干预措施至关重要.
研究的目的:
- 为了研究长非编码RNA (lncRNA) 和信使RNA (mRNA) 在特有 (EC) 中的差异性表达特征.
- 为了确定潜在的分子参与者和途径,涉及到EC的致病性.
- 在EC患者中探索lncRNAs和mRNAs之间的相互作用.
主要方法:
- 使用高通量RNA测序 (RNA-seq) 来分析lncRNA和mRNA的表达.
- 基因本体学 (GO) 和KEGG通路分析进行了功能注释.
- 蛋白与蛋白相互作用 (PPI) 网络构建和定量实时PCR (qRT-PCR) 被用于验证.
主要成果:
- 总共有864个lncRNA和393个mRNA被发现在EC患者和对照人群之间表达不同.
- 该PPI网络揭示了关键的蛋白质编码基因,其中LINC01220及其向mRNA IDO1在EC患者中显示了统计学上较高的水平.
- postsynaptic膜潜力的调节和Rap1信号通路与EC病理生理学有关.
结论:
- 差异表达的lncRNAs,特别是LINC01220,代表了EC.的病变发生过程中的潜在新型参与者.
- LINC01220和IDO1之间的相互作用可能有助于EC的发展.
- 这些发现为EC的基础分子机制提供了宝贵的见解.
相关概念视频
lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
Translation
14.7K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
Translation Produces the Building Blocks of Life
Proteins are...
14.7K
Types of RNA
5.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
5.7K
RNA Splicing
56.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.3K
Formation of Muscle Fibers from Myoblasts
4.9K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.9K


