季节性繁殖大西洋鱼的bmp15功能丧失导致排卵失败
Diego Crespo1, Per Gunnar Fjelldal2, Tom J Hansen2
1Research Group Reproduction and Developmental Biology, Institute of Marine Research, Bergen, Norway.
概括
骨形态遗传蛋白15 (BMP15) 对于女性生殖至关重要. 大西洋鱼bmp15突变体显示毛囊发育和排卵受损,为鱼类生育研究提供了一个新模型.
科学领域:
- 生殖生物学 生殖生物学
- 鱼类遗传学 鱼类遗传学
- 发育生物学是发展生物学.
背景情况:
- 骨形态遗传蛋白15 (BMP15) 对于哺乳动物雌性繁殖至关重要.
- 在哺乳动物中,BMP15突变会导致不孕.
- 斑马鱼bmp15淘汰赛显示卵泡停止和性别逆转,限制了排卵研究.
研究的目的:
- 研究Bmp15在大西洋鱼卵巢发育和生育能力中的作用.
- 在大西洋鱼中产生bmp15突变,以研究其在季节性繁殖场中的功能.
- 建立一个鱼类模型来研究Bmp15在无性别逆转的情况下在排卵中的作用.
主要方法:
- 生成的双变换 bmp15 (bmp15 fs/fs) 突变的大西洋鱼.
- 对卵巢进行了组织学和形态学分析.
- 测量了基因表达 (figla,star,cyp11b,cyp17a1,esr1) 和血中的雌激醇-17β水平.
- 评估性腺体指数,卵细胞直径,排卵,受精和胚胎发育.
主要成果:
- bmp15 fs/fs 卵巢表现出早期的卵泡发育和晚期发育,表达力较弱.
- 在bmp15 fs/fs雌性中,增强的卵泡缩发生在生性阶段.
- 尽管增加了类固醇基因表达,但在bmp15 fs/fs突变体中,雌醇水平较低.
- 雌性体腺指数降低,卵子小,排卵受损 (71.4%),受精问题或胚胎死亡率 (28.6%).
结论:
- 在大西洋鱼中,Bmp15对于适当的卵泡招募,生长和排卵成功至关重要.
- bmp15突变为研究鱼类排卵中的Bmp15功能提供了有价值的模型.
- 这个模型提供了一个潜在的目标,可以在不引起性别逆转的情况下诱导养殖鱼的不育.
相关概念视频
02:32Osmoregulation in Fishes
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
01:11Testing a Claim about Mean: Known Population SD
A complete procedure of testing the hypothesis about a population mean is explained here.
Estimating a population mean requires the samples to be distributed normally. The data should be collected from the randomly selected samples having no sampling bias. The sample size needed to be higher than 30, and most importantly, the population standard deviation should be already known.
In most realistic situations, the population standard deviation is often unknown, but in rare circumstances, when it...
Estimating a population mean requires the samples to be distributed normally. The data should be collected from the randomly selected samples having no sampling bias. The sample size needed to be higher than 30, and most importantly, the population standard deviation should be already known.
In most realistic situations, the population standard deviation is often unknown, but in rare circumstances, when it...