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Updated: Jun 20, 2025

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使用PhenoCycler-Fusion进行高复合性,全幻灯片成像的人类甲固定嵌组织的协议

Meg L Donovan¹, Niyati Jhaveri², Ning Ma²

¹Queensland Spatial Biology Centre, Wesley Research Institute, Level 8 East Wing, The Wesley Hospital, Auchenflower, QLD 4066, Australia; Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, QLD 4102, Australia.

|July 20, 2024

概括

这项研究详细介绍了使用PhenoCycler-Fusion 2.0技术进行高级单细胞空间蛋白质组分析的高复合性免疫光染色和人体头癌组织成像的自动化协议.

科学领域:

生物医学研究的研究.
癌症生物学癌症生物学
蛋白质组学是指蛋白质组学.

背景情况:

对蛋白质的单细胞空间分析对于理解生物机制至关重要.
影像技术的进步正在使更全面的蛋白质组研究成为可能.

研究的目的:

介绍自动化高复合多片免疫光染色和成像的详细协议.
为了能够对人类头癌组织进行详细的空间蛋白质组分析.

主要方法:

该协议使用PhenoCycler-Fusion 2.0技术进行自动染色和成像.
它涵盖组织制备,免疫型标记物染色,流细胞组装和数据采集.
此外,还概述了下游分析程序.

主要成果:

这项研究为高复杂空间蛋白质组分析提供了可重复的协议.
该方法适用于甲固定嵌 (FFPE) 的人类头癌部位.

结论:

该协议促进了FFPE组织的深入空间蛋白质基因分析.
它支持在癌症研究中推进单细胞空间分析.

关键词:

癌症癌症癌症癌症分子生物学分子生物学蛋白质组学是指蛋白质组学.一个单细胞单细胞.

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Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...