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一种强大的超高性能液态色谱-并联质谱法,用于同时确定谷氨酸循环中的10个成分

Xiang-Ling Qin¹, Si-Yu Wang¹, Qi-Long Li¹

¹Department of Pharmaceutical Analysis, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Journal of separation science

|July 20, 2024

概括

一种新的方法使用超高性能液体色谱-并联质谱来量化关键的谷氨 (GSH) 循环成分. 这项技术有助于理解氧化应激对细胞GSH通路的影响.

科学领域:

生物化学生物化学
分析化学分析化学
细胞生物学细胞生物学

背景情况:

谷氨 (GSH) 是一种关键的抗氧化剂,通过GSH循环合成和代谢.
精确量化GSH循环组件对于评估GSH代谢过程至关重要.
了解细胞对氧化应激的反应需要对GSH通路进行详细分析.

研究的目的:

开发和验证一种强大的超高性能液体染色学-并联质谱法 (UPLC-MS/MS) 方法.
在GSH循环中同时量化十个关键组件.
应用在正常与氧化应激条件下比较GSH循环概况的方法.

主要方法:

使用UPLC-MS/MS.同时量化十个GSH循环代谢物.
优化衍生物化 (n-乙烯胺),色谱分离 (氨基基柱,梯度化) 和光谱条件.
方法验证包括选择性,线性,精度,准确性,稳定性,恢复性和矩阵效应.

主要成果:

一种经过验证的UPLC-MS/MS方法实现了高选择性,线性 (R2>0.99) 和可接受的精度和准确性.
该方法证明了针对目标GSH循环组件的良好稳定性,恢复性和最小的矩阵效应.
开发的试验成功地用于区分正常和氧化应激诱导细胞之间的GSH循环成分水平.

结论:

该UPLC-MS/MS方法提供了一个可靠的工具,用于同时定量多个GSH循环代谢物.
这种验证的测定方法适用于比较研究,特别是评估细胞对氧化应激反应.
这些发现有助于更深入地了解氧化压力如何影响GSH循环的复杂生化途径.

关键词:

这是GSHGSH.在UPLC-MS/MS中使用.葡萄糖氨酸是什么葡萄糖氨酸谷氨二的循环周期氧化应激是一种氧化应激.

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