在极端友藻类,Galdieria spp.的连续培养过程中对基质和营养物质进行操纵. RTK 37.1,对生物质的生产率和组成有重大影响
Emma Buckeridge1, Carlos C Caballero1,2,3, Daniel H Smith1
1Te Tari Pūhanga Tukanga Matū, Department of Chemical and Process Engineering, Te Whare Wānanga o Waitaha, University of Canterbury, Christchurch, Aotearoa, New Zealand.
Biotechnology and bioengineering
|July 20, 2024
概括
这项研究表明Galdieria sp. 在各种条件下,RTK371藻类可以连续培养. 在种植过程中操纵营养水平可以控制生物质成分,产生碳水化合物或蛋白质.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 藻类生理学 藻类生理学
背景情况:
- 盖尔迪埃里亚公司 (Galdieria Spp.) 它们是具有代谢灵活性的极端友藻类,表现出生物技术潜力.
- 关于连续种植的有限研究阻碍了Galdieria spp.的商业化.
研究的目的:
- 研究Galdieria sp.的生物质生产率和生长产量. 在连续光自otrophic,混合otrophic 和异质otrophic 种植下RTK371.
- 确定Galdieria sp.的最佳生长条件和产品产量. RTK371.一个人的世界
主要方法:
- 连续种植的Galdieria sp. 的种植. 在不同的光和营养条件下,RTK371在化学静止剂中.
- 生物质生产率,生长产量,氧气生产和细胞内成分 (碳水化合物,蛋白质) 的监测.
主要成果:
- 盖尔迪埃里亚 (Galdieria) 公司 RTK371 在pH2.5和100μmol m−2 s−1照明下增长最佳.
- 在不同种植方式中,氧气的产生有显著的差异:光自otrophic (133.5 nmol O2 mg−1 h−1),混合otrophic (42 nmol O2 mg−1 h−1),和异质otrophic (<0.01 nmol O2 mg−1 h−1).
- 在所有方案中都实现了稳定的长期增长;氨限制增加了碳水化合物含量 (高达37% w/w),而过量的氨则产生了高蛋白质含量 (高达65% w/w).
结论:
- 连续种植的Galdieria sp. 的种植. RTK371在光自otrophic,混合otrophic 和异质otrophic 条件下是可行的.
- 可以操纵生长条件以控制生物质成分,从而实现有针对性的碳水化合物或蛋白质生产.
- 这项研究支持了Galdieria spp.的商业化潜力. 通过优化的连续种植策略.
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