PP2C酸酶Pic14通过抑制MAPK激活来负面调节番茄Pto/Prf触发的免疫力
Joydeep Chakraborty1, Guy Sobol1, Fan Xia2,3
1School of Plant Sciences and Food Security, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel.
The Plant journal : for cell and molecular biology
|July 20, 2024
概括
2C型蛋白酸酶 (PP2Cs) 调节植物免疫力. PP2C基因Pic14对番茄产生负面影响.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 信号传导传导是指信号的传导.
背景情况:
- 2C型蛋白酸酶 (PP2Cs) 是植物免疫反应的关键调节者.
- PP2Cs在核酸结合,富含白素的重复 (NLR) 触发免疫 (NTI) 中的作用尚不清楚.
- 番茄中Pto/Prf介导的NTI通路赋予了对Pseudomonas syringae pv.的耐药性. 番茄 (Pst) 通过一个MAPK级联.
研究的目的:
- 研究PP2C基因Pic14在番茄Pto/Prf介导的NTI反应中的作用.
- 阐明 Pic14 调节植物免疫力的分子机制.
主要方法:
- 在Pst-AvrPto注射后对Pic14的基因表达分析.
- 在番茄中的Pic14中产生和分析功能丧失突变.
- 在Nicotiana benthamiana和番茄中进行过渡表达测试,以评估Pic14的功能.
- 生物化学测试以确定Pic14的酸酶活性和与Mkk2.2的相互作用.
主要成果:
- Pic14表达是由Pto/Prf介导的NTI反应诱导的.
- Pic14中的功能丧失突变增强了番茄对Pst细菌斑点病的抵抗力.
- 皮克14抑制了Pto/Prf和MAPK诱导的细胞死亡.
- 皮克14以依赖酶相互作用动机 (KIM) 的方式去酸化Mkk2,抑制MAPK激活.
结论:
- 皮克14作为西红中Pto/Prf触发免疫力的负调节剂.
- 皮克14通过与Mkk2相互作用和脱,从而抑制MAPK级联而起作用.
- 了解Pic14的作用可以了解植物免疫信号的复杂调节.
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