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Updated: Jun 20, 2025

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暴露诱导了Arabidopsis植物在亚细胞水平上的光/黑暗和时间依赖的氧化还原变化

Aurelio M Collado-Arenal¹, Marino Exposito-Rodriguez², Philip M Mullineaux²

¹Department of Biochemistry and Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, CSIC, Granada 18008, Spain.

Journal of hazardous materials

|July 20, 2024

概括

暴露于会在植物细胞中引起氧化应激,影响诸如质体和过氧体之类的有机体. 光和光合成电子运输链活动影响了这种引起的损伤.

科学领域:

植物生物学植物生物学
环境毒理学环境毒理学
细胞再氧化生物学细胞再氧化生物学

背景情况:

(Cd) 是一种高度有毒的重金属,对植物和人类产生影响.
活性氧物种 (ROS) 是植物对Cd反应中的关键信号分子,但它们的亚细胞起源和依赖光的动态仍然不清楚.

研究的目的:

为了研究对Arabidopsis thaliana内的不同器官中的细胞氧化还原状态的影响.
为了确定光条件和光合作用电子运输链 (pETC) 活动对Cd诱导的氧化应激的影响.

主要方法:

利用表达区特定GRX1-roGFP2传感器的Arabidopsis线来监测氧化还原状态的变化.
在Cd处理下,在24小时的光/黑暗周期中,分析了叶片盘的过氧体和叶片的氧化还原状态.
研究了DCMU (一种pETC抑制剂) 的作用和塑性NTRC (NADPH thioredoxin减少酶C) 的沉默.

主要成果:

在所有分析的有机体中诱导了时间和光线依赖的氧化增加.
叶绿体和氧体受到显著影响,氧体显示出快速的氧化还原变化.
抑制pETC和静止NTRC加剧了Cd依赖氧化,特别是在过氧体中.
在Cd暴露期间观察到NAD,P) H和细胞质NADH的减少,以及质质/细胞质Ca+2的增加.

结论:

植物细胞中诱导的氧化是一个复杂的过程,受到光,pETC功能和光呼吸的影响.
抗氧化能力和辅助因子可用性的亚细胞差异有助于器官特异性对压力的反应.
了解这些氧化还原动力学对于减轻植物中的毒性至关重要.

关键词:

是的组成部分.在GRX1-roGFP2中使用.有机器人有机器人有反应性氧物种的反应性氧物种.氧化还原平衡 (redox homeostasis) 是一种方法.

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