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通过人类肠道细菌Bacteroides eggerthii的三个酶对酸盐降解的行动和合作 DSM 206977 DSM 20697

Mette E Rønne¹, Christian Dybdahl Andersen², David Teze³

¹Enzyme and Protein Chemistry, Department of Biotechnology and Biomedicine, Technical University of Denmark, Lyngby, Denmark; Norwegian Biopolymer Laboratory (NOBIPOL), Department of Biotechnology and Food Science, NTNU Norwegian University of Science and Technology, Trondheim, Norway.

The Journal of biological chemistry

|July 20, 2024

概括

肠道细菌,如 Bacteroides eggerthii,可以使用特定的酶来分解海藻多糖酸盐 - - 酸盐. 这项研究详细介绍了这些酶在酸盐降解过程中的分子机制和它们之间的合作.

科学领域:

微生物学和生物化学
碳水化合物的新陈代谢.
酶学是一种酶学.

背景情况:

酸盐是一种来自海藻的多糖,用于食品和医药.
肠道细菌可以将酸盐发酵成有益的短链脂肪酸,但分子细节尚不清楚.
细菌卵菌 DSM 20697 具有用于酸盐分解的多糖化物利用位点 (PUL).

研究的目的:

为了阐明由 Bacteroides eggerthii 的酸盐降解的分子机制.
描述参与酸盐分解的关键酶的作用和合作.
研究BeKdgF金属蛋白的结构和催化机制.

主要方法:

生物化学试验以确定酶的特异性和活性 (BePL6,BePL17,BeKdgF).
进行X射线晶体学以确定BeKdgF.的三维结构.
通过NMR的pH定位来确定BeKdgF中关键氨基酸残留的pKa.

主要成果:

BePL6和BePL17是外作用的多糖酸酶,分别具有氨酸 (G) 和曼努伦酸 (M) 块的特异性.
一种金属蛋白BeKdgF催化不和单酸盐的环开放,并在EDTA存在时保持部分活性.
BePL6,BePL17和BeKdgF的合作作用增强了酸盐的降解,进一步由外部内作用酸盐酶促进.

结论:

细菌卵菌利用一个协同作用的酶系统,涉及BePL6,BePL17和BeKdgF进行酸盐降解.
对BeKdgF的结构和机制洞察力,为我们提供了对多糖分解路径的更深入的理解.
这些发现有助于了解肠道中藻酸盐的微生物利用.

关键词:

这是一种类似KdgF的酶.酸是一种酸.阿尔基酸酸酶的使用碳水化合物加工加工的方法酶动力学酶动力学酶机制的酶机制是什么人的肠道微生物群聚糖的利用位置是多糖的利用位置.蛋白质结构蛋白质结构

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