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过度表达PRDM16可以改善旋转手套撕裂后的肌肉功能

He Zhang¹, Aboubacar Wague², Agustin Diaz²

¹Department of Physical Education, Central South University, Changsha, Hunan, China; Department of Veterans Affairs, San Francisco Veterans Affairs Medical Center, San Francisco, CA, USA; Department of Orthopedic Surgery, University of California, San Francisco, San Francisco, CA, USA.

Journal of shoulder and elbow surgery

|July 20, 2024

概括

升高的PRDM16水平通过增强肌肉功能和减少脂肪透,改善了旋转手套撕裂 (RCT) 的结果. 这表明,促进棕色脂肪组织 (BAT) 活动可以帮助RCT后的肩膀功能恢复.

科学领域:

生物医学研究的研究.
肌肉和脂肪组织生物学
再生医学是一种再生医学.

背景情况:

旋转手套撕裂 (RCT) 经常导致肌肉缩,纤维化和脂肪透,对患者的治疗结果产生负面影响.
在RCT后改善肌肉质量对于成功修复肌和功能恢复至关重要.
功能性色/棕色脂肪组织 (BAT) 分泌可以促进肌肉生长的巴托基因,PRDM16是棕色脂肪发育的关键调节者.

研究的目的:

为了研究PRDM16在大规模肌撕裂后增强肌肉功能的作用.
利用具有高PRDM16表达的转基因小鼠模型研究其对旋转手套肌肉质量影响.

主要方法:

转基因PRDM16过度表达的小鼠和野生类型的对照小鼠经历了旋转手套和上神经切割 (TTDN).
前肢功能在受伤后6周使用DigiGait进行评估.
肌肉纤维化,脂肪透和纤维类型 (MHC表达) 分别通过Masson Trichrome,Oil Red O和免疫染进行分析.

主要成果:

在白色和棕色脂肪组织中,PRDM16过度表达增加了PRDM16蛋白.
尽管肌肉重量没有变化,但PRDM16过度表达显著改善了前肢功能 (更长的姿势,步伐时间,更大的步伐长度/脚面积).
脂肪透显著减少,而纤维化保持不变;在上肌肉中观察到MHC-IIx纤维百分比增加.

结论:

过度表达PRDM16显著提高肌肉功能,并减少旋转手套撕裂后的脂肪透.
激活棕色脂肪组织 (BAT) 有望改善旋转手套肌肉质量和RCT后的肩部功能.

关键词:

旋转器袖口撕裂的眼色的胖子色的胖子脂肪的透脂肪的透纤维基原始体的原始体纤维化纤维化肌肉缩肌肉缩肌肉缩

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