在常见鱼 (Cyprinus carpio) 中,CcPTGS2a类基因介导的NF-κB/ERK信号调节
Feng Gao1, Yanjing Zhao1, Yunli Zhang1
1Engineering Lab of Henan Province for Aquatic Animal Disease Control, College of Fisheries, Henan Normal University, Henan Province, PR China.
Fish & shellfish immunology
|July 20, 2024
概括
研究人员在普通鱼中发现了一种类似CcPTGS2a的基因,该基因可以增强对细菌感染的先天免疫力. 这种基因的过度表达通过激活NF-κB/ERK信号通路来提高鱼的生存率.
科学领域:
- 免疫学 免疫学 免疫学
- 鱼类生物学 鱼类生物学
- 分子生物学分子生物学
背景情况:
- 前列腺素内氧化合成酶2 (PTGS2) 在先天免疫和病原体识别中起着至关重要的作用.
- 了解鱼类的免疫反应对于水产养殖和疾病管理至关重要.
研究的目的:
- 为了识别和表征常见鱼 (Cyprinus carpio) 中的CcPTGS2a类基因.
- 为了研究CcPTGS2a-like在对Aeromonas hydrophila感染的免疫反应中的作用.
- 阐明NF-κB/ERK信号通路在CcPTGS2a类似介导免疫中的参与.
主要方法:
- 对CcPTGS2a类似的基因鉴定和序列分析.
- 细菌挑战后在各种组织中对mRNA和蛋白质表达的定量分析.
- 细菌结合测定和鱼类生存率评估.
- 基于细胞的测试分析信号通路激活 (NF-κB/ERK) 和细胞因子表达.
主要成果:
- 在普通鱼中发现了一种具有表皮生长因子和过氧化酶域的CcPTGS2a类基因.
- 在Aeromonas hydrophila刺激后,免疫组织中的CCPTGS2a类表达被上调.
- 通过减少细菌负载,CcPTGS2a类似的细菌结合活性和改善鱼存活率.
- CcPTGS2a类似调节NF-κB/ERK通路,影响关键信号分子和炎症性细胞因子表达.
结论:
- CcPTGS2a-like 是普通鱼天生的免疫系统的重要组成部分.
- 该基因对细菌感染起着保护作用,可能通过NF-κB/ERK信号通路.
- 类似于CcPTGS2a的水产养殖物种是提高水产养殖中鱼类抗病能力的潜在目标.
相关概念视频
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
MAPK Signaling Cascades
5.4K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.4K
TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K


