Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Coagulation01:06

Coagulation

280
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
280

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Metaproteomics for Water Biotechnology: Considerations and Study Cases.

Advances in experimental medicine and biology·2026
Same author

Parabens at Environmental Levels Modulate Virulence and Antimicrobial Tolerance of Exposed Biofilm Cells.

Antibiotics (Basel, Switzerland)·2026
Same author

Ultrastructure Analysis by Cryo-Electron Tomography Revealed Mesosomes in the Gram-negative Delftia Acidovorans.

Microbial ecology·2026
Same author

The action of selected monoterpenes as biofilm control agents and antibiotic resistance modifiers.

Biofouling·2026
Same author

Protocol to study bacterial coaggregation by integrating visual, turbidimetry, microscopy, and thermodynamic analysis.

STAR protocols·2025
Same author

Cell phenotyping reveals chemical water disinfection treatment-induced molecular and morphological perturbations.

Journal of hazardous materials·2025

相关实验视频

Updated: Jun 20, 2025

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

723

通过物理化学和成像表面表征来阐明细菌凝聚.

Ana C Afonso1, Jack Botting2, Inês B Gomes3

  • 1LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal; ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal; CITAB, Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal; CEB-LABBELS, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

The Science of the total environment
|July 20, 2024
PubMed
概括

在水生系统中,细菌的凝聚是由Delftia acidovorans 005P.上的像 pili 的粘合物驱动的. 这些独特的结构,以及表面特性,调解细胞与细胞相互作用,这对生物膜形成和水质至关重要.

关键词:
细胞与细胞的相互作用.共同凝聚力能源 凝聚力能源低温电子断层扫描 (Cryo-electron tomography) 是一种电子断层扫描技术.德尔夫酸的发言人美国的FTIR是FTIR.在XDLVO理论中,

更多相关视频

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.1K
Biomimetic Materials to Characterize Bacteria-host Interactions
12:22

Biomimetic Materials to Characterize Bacteria-host Interactions

Published on: November 16, 2015

9.3K

相关实验视频

Last Updated: Jun 20, 2025

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry

Published on: September 29, 2023

723
Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
06:51

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Published on: August 2, 2018

7.1K
Biomimetic Materials to Characterize Bacteria-host Interactions
12:22

Biomimetic Materials to Characterize Bacteria-host Interactions

Published on: November 16, 2015

9.3K

科学领域:

  • 微生物学 微生物学
  • 表面化学 表面化学
  • 生物物理学的生物物理.

背景情况:

  • 细菌凝聚,一种特定的细胞-细胞相互作用,在口腔细菌中很好地理解,但在水生环境中表征不佳.
  • 了解凝聚机制对于其对生物膜,水质,工程系统和生物技术的影响至关重要.
  • 这项研究调查了导致Delftia acidovorans,饮用水分离物的凝聚的因素.

研究的目的:

  • 综合性地描述一个凝聚菌株 (Delftia acidovorans 005P) 和一个非凝聚菌株 (D. acidovorans 009P) 的细胞表面.
  • 确定负责005P菌株凝聚能力的特定表面因子.

主要方法:

  • 细菌表面性质的物理化学表征.
  • 福利埃变换红外光谱 (FTIR) 用于化学分析.
  • 传输电子显微镜 (TEM) 和冷电子断层扫描 (cryo-ET) 用于细胞结构的高分辨率成像.

主要成果:

  • 与非凝聚性菌株 (009P) 相比,凝聚性菌株 (005P) 显示出更高的表面疏水性,负面电荷和凝聚力能量.
  • FTIR揭示了菌株之间的碳水化合物和蛋白质 (基/胺基III) 的微妙差异.
  • 化ET检测发现了 pili 结构的显著变异,其中005P 具有更频繁,更明显的 pili 类粘合素.

结论:

  • Delftia acidovorans 005P 的凝聚能力与其独特的像 pili 的粘合素直接相关.
  • 这些 pili 作为水生环境中细胞与细胞相互作用的关键媒介.
  • 这项研究为水系统中的微生物凝聚机制提供了关键的见解.