来自活体和热杀死的囊多糖的结构特征和免疫活性 Lacticaseibacillus paracasei 6235
Yucong Wang1, Rongxu Liu2, Zhixin Xie1
1College of Food Science, Northeast Agricultural University, Harbin 150030, China.
International journal of biological macromolecules
|July 20, 2024
概括
与活体CPS相比,来自Lacticaseibacillus paracasei 6235的热杀囊多糖 (HCPS) 显示出增强的免疫调节作用. HCPS更有效地降低了促炎性细胞因子,并调节了免疫路径,表明了后生物发展的潜力.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 生物化学 生物化学
背景情况:
- 益生菌中的囊多糖 (CPS) 可以调节宿主免疫功能.
- 热杀死CPS (HCPS) 的结构和功能变化尚未得到充分理解.
- 研究HCPS为开发新型后生物产品提供了潜力.
研究的目的:
- 为了比较活体CPS (LCPS) 和来自Lacticaseibacillus paracasei的HCPS之间的结构和免疫调节差异6235.
- 分析热处理对CPS结构的影响及其随后的免疫效应.
- 评估HCPS作为后生物成分的潜力.
主要方法:
- 隔离和净化CPS从活的和热杀死的乳酸酶菌 (Lacticaseibacillus paracasei) 6235.
- 使用甲基化,核磁共振和单糖化合物组成的结构分析.
- 在LPS诱导的RAW264.7细胞中的体外免疫调节评估,测量细胞因子水平和信号通路激活 (NF-κB,MAPK).
主要成果:
- 热杀死降低了CPS分子量,改变了单糖化合物的组成 (失去银胺).
- 无论LCPS还是HCPS都表现出类似的核心结构,但HCPS显示IL-6和IL-1β的下调增强.
- 与LCPS相比,HCPS对NF-κB和MAPK信号通路的影响更大.
结论:
- 热处理改变了CPS结构,导致增强了免疫调节活性.
- 在通过NF-κB和MAPK通路减轻炎症反应方面,HCPS比LCPS更有效.
- 这些发现支持HCPS作为一种强大的免疫调节后生物的发展.
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