歇斯酸乳化-ROS循环有助于斑马鱼中暴露在光线下加剧的中性粒细胞的招募
Cheng-Zeng Qiu1, Ren Zhou1, Hao-Yi Zhang1
1College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, China.
Communications biology
|July 20, 2024
概括
被破坏的光周期通过基因素乳化-ROS反循环增加了中性粒细胞的招募. 保持光暗周期对于最佳免疫功能和预防过度炎症至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 光是一种关键的环境暗示,调节生理恒温.
- 被破坏的光暗周期会损害免疫功能,导致炎症加剧.
- 将光干扰与免疫失调联系在一起的分子机制尚未完全理解.
研究的目的:
- 调查持续暴露于光线对斑马鱼中中性粒细胞招募的影响.
- 阐明分子通路,包括质母乳化和反应性氧物种,参与光诱导炎症.
主要方法:
- 在斑马鱼的炎症模型的体内成像.
- 使用ChIP测序和ChIP-qPCR分析基因组修饰和基因调节.
- 测量歇斯顿乳酸和活性氧物种 (ROS) 水平.
主要成果:
- 持续照明 (LL) 条件显著增加了对炎症部位的中性粒细胞的招募.
- 对LL的暴露导致了素 (H3K18) 乳酸和ROS水平的升高.
- 确定了H3K18乳酸和ROS之间的积极反循环,调节双基因表达并促进中性粒细胞过度招募.
结论:
- 一个新的H3K18乳化-ROS反循环在光障碍条件下加剧了中性粒细胞的招募.
- 这项研究强调了光暗周期在调节免疫反应中的关键作用.
- 保持适当的光暗周期对于优化免疫功能和预防炎症失调至关重要.
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