探索肌球蛋白中的结构和计算对比:对热处理诱导的硫肌球蛋白形成的影响
Juan C Ramírez-Suárez1, Andrés Álvarez-Armenta1, Jose Angel Huerta-Ocampo2
1Laboratorio de Bioquímica y Calidad de Productos Pesqueros, Centro de Investigación en Alimentación y Desarrollo, A.C., 83304, Hermosillo, Sonora, Mexico.
Food chemistry
|July 21, 2024
概括
鱼甲基球蛋白 (MetMb) 变绿与硫基球蛋白 (SulfMb) 有关. 这项研究表明,GR无反应的MetMb,就像Sarda chiliensis一样,可以防止由于Met85在热处理过程中破坏基团的稳定而导致硫Mb的形成.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
背景情况:
- 通过热处理 (TT) 的鱼甲基球蛋白 (MetMb) 的绿化和囊蛋白形成硫基球蛋白 (SulfMb).
- 这种绿化反应 (GR) 以前被认为仅限于金枪鱼,但也发生在马群中.
- 存在变化,一些金枪鱼物种和马MetMb显示GR不活性.
研究的目的:
- 为了比较不同物种的GR反应性和GR无反应性MetMb.
- 为了研究MetMb绿化背后的分子机制.
- 在热处理过程中分析血质稳定性.
主要方法:
- 在TT (60°C/30分钟) 期间使用自由氨酸监测绿化反应的紫外线视频谱学.
- 分子动力学 (MD) 模拟以评估血红团稳定性.
- 来自Katsuwonus pelamis (GR-反应性),Equus caballus (GR-反应性),Sarda chiliensis (GR-无反应性) 和Euthynnus lineatus (GR-无反应性) 的MetMb的比较.
主要成果:
- GR-无反应的MetMb (Sarda chiliensis,Euthynnus lineatus) 显示不完全的GR,没有生产硫.
- 模拟MD显示Met85在GR无反应的heme腔中的MetMb导致heme不稳定.
- 在TT过程中,Met85的存在导致远端胺的位移.
结论:
- Met85在血红腔中的作用对MetMb的GR不反应性至关重要.
- 血红组的不稳定性和远端胺的位移解释了在某些MetMb中缺乏硫基的形成.
- 了解这些机制是控制肉的颜色和质量的关键.
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