将高性能星形双金属纳米标签引入SERS感应传感器:一种超敏感且无干扰的方法来检测pyrifos
Hui Ma1, Lingmeng Hu1, Fangchen Ding1
1College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
Biosensors & bioelectronics
|July 21, 2024
概括
开发了一种新的表面增强拉曼散射 (SERS) 适应传感器,用于检测pyrifos (CPF) 农药残留物. 这项技术可以在农产品中提供超敏感,可靠和无干扰的检测,从而提高食品安全.
科学领域:
- 分析化学 分析化学
- 纳米技术 纳米技术
- 食品安全 食品安全
背景情况:
- 越来越多的食品安全问题需要对农药残留物进行敏感,快速和准确的检测.
- 现有的农药检测方法经常面临成本效益,可靠性和效率方面的挑战.
- 表面增强拉曼散射 (SERS) 为高度敏感的分子检测提供了潜力.
研究的目的:
- 引入一个高性能SERS吸食传感器,用于超敏感检测pyrifos (CPF).
- 开发一种可靠且无干扰的方法,用于农产品中的农药残留分析.
- 利用纳米标签技术与磁性分离相结合,以简化样品处理.
主要方法:
- 使用星形双金属纳米标签 (AuNS@4-MBN@Ag-aptamer) 作为信号增强材料制造SERS适应传感器.
- 包括4-mercaptobenzonitrile (4-MBN) 作为一个"生物静音"窗口记者在2228厘米-1.
- 利用与cDNA结合的Fe3O4@AuNPs (FA-cDNA) 作为磁基质,以有效地分离纳米标记混合物.
主要成果:
- 胃感应器显示CPF的广泛线性检测范围从2.5×102到5.0×104 pg·mL−1.1.
- 取得了令人印象深刻的220.35 pg·mL−1 (S/N = 3) 的低检测极限.
- 在小麦和果的实际应用中显示了令人满意的尖端回收率 (89.61%107.33%),具有良好的可复制性和选择性.
结论:
- 开发的基于纳米标签的SERS吸食传感器提供了一个高度敏感,可靠和无干扰的平台,用于痕迹CPF检测.
- 使用磁基板简化了分离过程,使该方法对复杂矩阵更加实用.
- 这种口味传感器代表了加强农产品中农药残留物食品安全监测的有希望的工具.
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