相关实验视频

Updated: Jun 20, 2025

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

脂质积累驱动多巴胺基神经元中的细胞衰老

Taylor Russo^1,2, Markus Riessland^1,2

¹Department of Neurobiology and Behavior, Stony Brook University, Stony Brook, NY 11794, USA.

|July 21, 2024

概括

大脑细胞中的脂质积累和细胞衰老有助于帕金森病 (PD) 的进展. 解决脂质积累可能为这种神经退行性疾病提供新的治疗策略.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

帕金森病 (PD) 涉及多巴胺基 (DA) 神经元的损失,但其原因尚不清楚,缺乏有效的治疗方法.
溶解体功能障碍和脂质积累,包括α-synuclein和葡萄糖核糖体 (GluCer),都与PD进展有关.
一个常见的PD危险因子GBA基因编码了对脂质代谢至关重要的溶酶酶 (GCase).

研究的目的:

研究脂质积累和细胞衰老在PD中DA神经元退化中的作用.
探索 lysosomal 损伤,脂质聚合,细胞衰老和PD 发病过程中的炎症之间的联系.
为了检查衰老的DA神经元中脂质滴相关蛋白的表达.

主要方法:

在DA神经元中诱导葡萄糖脑化物 (GluCer) 积累.
对DA神经元中细胞衰老标记物的分析.
在衰老的DA神经元中评估利平 (PLIN2) 表达.
审查关于脂质代谢,溶酶体功能和神经退行现有的文献.

主要成果:

人工GluCer积累诱导DA神经元中的细胞衰老.
易受伤害的DA神经元中的细胞衰老表型可以由溶酶体损伤和脂质聚合触发.
在衰老的DA神经元中,Perilipin 2 (PLIN2) 被上调调节,这表明脂质滴滴丰富.

结论:

与年龄相关的脂质聚合和溶酶体功能障碍有助于DA神经元中的细胞衰老.
细胞衰老和中脑中相关的炎症是PD中神经退行的主要驱动因素.
准脂质积累途径为帕金森病提供了潜在的治疗途径.

关键词:

帕金森病是帕金森氏症的一种疾病.细胞衰老细胞衰老葡萄糖基胺的含量是多少?脂质脂质脂质是一种溶解体是溶解体的组成部分.神经炎症是一种神经炎症.

更多相关视频

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Author Spotlight: Generating Neuronal Phenotypic Profiles - A Protocol to Culture and Image Human Midbrain Dopaminergic Neurons

Published on: July 7, 2023

Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons

Studying Pre-formed Fibril Induced α-Synuclein Accumulation in Primary Embryonic Mouse Midbrain Dopamine Neurons

Published on: August 16, 2020

相关概念视频

Parkinson's Disease: Overview

Parkinson's Disease: Overview

Neurodegenerative disorders are progressive diseases that cause irreversible damage and loss to neurons in specific brain areas. Examples of these disorders include Parkinson's disease, Alzheimer's disease, Multiple Sclerosis (MS), and Amyotrophic Lateral Sclerosis (ALS). These disorders share characteristics such as proteinopathies, selective neuronal vulnerability, and a complex interplay between genetic and environmental factors. The primary therapeutic goal for these conditions is...

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...