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Updated: Jun 20, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

鉴定和功能分析与肺中的氨暴露相关的循环RNA表达特征

Yuyi Ma¹, Qingtao Gu¹, Xinqi Cao¹

¹College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.

|July 21, 2024

概括

这项研究揭示了暴露于氨的肺中的循环RNA (circRNA) 表达变化,确定了参与免疫反应对这种呼吸系统污染物的关键调节网络.

科学领域:

环境毒理学环境毒理学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

氨是一种有害的空气污染物,会损害呼吸系统的健康.
循环RNAs (circRNAs) 是非编码RNAs,在生物过程中发挥作用,但它们在氨诱导的肺免疫中的功能尚不清楚.

研究的目的:

在暴露于氨水后,研究肺中的circRNA表达特征.
识别参与对氨的免疫反应的circRNA及其调节网络.

主要方法:

用RNA测序 (RNA-seq) 来分析暴露于氨的肺中的circRNA表达.
鉴定了差异表达的circRNA,并分析了它们的源基因以进行途径丰富.
使用RT-qPCR构建和验证了circRNA-miRNA-mRNA调节网络.

主要成果:

鉴定了61种差异表达的环RNA,其中17种是上调调的,44种是下调调的.
丰富的途径包括A型流感,SNARE相互作用和Notch信号传递.
经过验证的circRNA和构建的调节网络,例如circRAB11A-gga-miR-191b-3p-BRD2和circARID3B-gga-miR-1696-CKS2.2.

结论:

这项研究提供了第一个全面的circRNA表达特征在肺在氨压力下.
已识别的circRNA和调节网络为氨诱导的呼吸道免疫反应的分子机制提供了洞察力.
这些发现可能有助于理解和减轻人类和动物因氨污染引起的疾病.

关键词:

氨气氨气氨气是一种肉肉肉肉在RNA-seqqq.监管网络监管网络是指监管网络的组成部分.环RNA 环RNA 是一个环RNA.

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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