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在妊娠10-14周的血清中miRNAs与自发早产之间存在关联

Tove Wikström^1,2, Sung Hye Kim^3,4, Anna-Lena Leverin¹

¹Department of Obstetrics and Gynecology, Center of Perinatal Medicine and Health, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Acta obstetricia et gynecologica Scandinavica

|July 22, 2024

概括

像miR-93-5p,miR-15b-5p和miR-191-5p这样的特定血清微RNA (miRNA) 与一般人群中自发早产 (PTD) 有关. 这些发现表明,预测PTD风险的潜在新生物标志物.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
发现生物标志物的发现.

背景情况:

过早分娩 (PTD) 是婴儿死亡的主要原因.
超声波检测到的宫缩短有助于PTD预测,但需要补充标记物.
怀孕早期的特定血微RNA (miRNA) 在高风险群体中显示出对PTD预测的希望.

研究的目的:

在一般人群中调查特定血清miRNA和自发PTD之间的关联.
探索这些miRNA与子宫长度之间的关系.
在更广泛的人口群体中验证之前观察到的miRNA关联.

主要方法:

在CERVIX队列中使用了一个嵌套病例控制研究设计.
分析了来自自发PTD (<34周) 的妇女的血清样本和全程分娩对照.
用RT-qPCR量化9种特定miRNA的表达水平.

主要成果:

在PTD<34周和满期分娩之间没有发现显著的miRNA差异.
在<32周的分娩中,MiR-191-5p和miR-93-5p显著过度表达,表达的增加与更早的妊娠年龄相关.
与对照组相比,在分娩30周以下的妇女中,MiR-15b-5p显著升高.

结论:

在妊娠早期血清中,miR-93-5p,miR-15b-5p和miR-191-5p的过度表达与一般人群中自发性PTD有关.
这些miRNAs显示出未来自发PTD生物标志物的潜力.
需要进一步的研究来证实它们的有用性,并阐明它们在PTD病理生理学中的作用.

关键词:

这是一个微型RNA.测量子宫长度的测量基因表达特征基因表达特征产妇血清查试验是对母体血清进行查的.过早出生过早出生

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...