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Updated: Jun 20, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

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Published on: June 2, 2021

一种新的MAP激酶相互作用蛋白MoSmi1调节了Magnaporthe oryzae中的发育和病原性

Yu Wang^1,2, Xinyue Cui^1,2, Junlian Xiao^1,2

¹Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, China.

Molecular plant pathology

|July 22, 2024

概括

这项研究揭示了MoSmi1对于Magnaporthe oryzae的真菌细胞壁完整性和病原性至关重要. 删除MoSmi1会损害压缩管功能和MAPK信号传递,影响真菌的发展.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
植物病理学植物病理学

背景情况:

菌细胞壁对于维持生理功能和抵抗外部压力至关重要.
莫纳普1是Magnaporthe oryzae中的核细胞组合蛋白,影响细胞壁完整性 (CWI),应激反应和致病性.
MoNap1负面调节了MoSMI1.1的表达.

研究的目的:

为了研究MoSMI1在Magnaporthe oryzae中的功能.
阐明MoSmi1在调节细胞壁完整性,发育和致病性方面的作用.
了解MoSmi1功能背后的分子机制,特别是它与信号通路的相互作用.

主要方法:

在Magnaporthe oryzae中删除了MoSMI1的基因.
对MoSMI1删除突变体中压缩体功能,细胞形态和致病性的分析.
蛋白质相互作用研究 (MoSmi1与MoOsm1和MoMps1的MoSmi1).
对关键MAPK路径组件 (MoOsm1,MoMps1,MoPmk1) 的酸化水平分析.
转录组分析以确定受监管的过程.

主要成果:

删除MoSMI1导致压缩体功能,CWI,细胞形态和致病性显著缺陷.
MoSmi1与MoOsm1和MoMps1相互作用,影响MoOsm1,MoMps1和MoPmk1.1的酸化.
转录组数据表明,MoSmi1调节了与膜相关的通路和氧化-减氧过程.
MoSmi1在调解基因激活蛋白激酶 (MAPK) 信号通路方面发挥着至关重要的作用.

结论:

在Magnaporthe oryzae中,MoSmi1对于维持细胞壁完整性和致病性至关重要.
MoSmi1通过调解MAPK信号通路来调节CWI和真菌发育.
这些发现突出了MoSmi1作为M. oryzae感染相关过程的关键调节者.

关键词:

压缩物形成的形成.细胞壁的完整性细胞壁的完整性病原性病原性病原性监管监管监管监管监管监管米爆炸真菌米爆炸真菌

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