相关实验视频

Updated: Jul 26, 2026

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Optical Clearing and Imaging of Immunolabeled Kidney Tissue

Published on: July 22, 2019

优化精密切割的片的长期化

C Hoeffner¹, F Worek¹, G Horn¹

¹Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.

Toxicology mechanisms and methods

|July 22, 2024

概括

为精密切割片 (PCKS) 优化化条件至关重要. 一种DMEM/F-12介质和 gentamicin 抗生素的组合最能支持PCKS的活力,长达10天.

科学领域:

药理学和毒理学药理学和毒理学
细胞生物学细胞生物学
脏生理学脏生理学

背景情况:

精密切割的脏切片 (PCKS) 是有价值的研究模型,可以弥合体内和体外研究.
现有的文献缺乏PCKS的标准化化协议,导致结果变化.
为了获得最佳的PCKS培养,需要比较不同的介质和抗生素组合.

研究的目的:

为了标准化和优化大鼠PCKS的化条件.
确定最有效的介质和抗生素组合,以保持PCKS的活力.
评估评估短期和长期PCKS可行性的方法.

主要方法:

鼠PCKS被用九种不同的介质和抗生素组合化了四天.
用定性和定量方法评估细胞活力,包括活体/死体染色,总蛋白质含量分析和乳酸脱酶 (LDH) 测定.
用标志性的蓝细胞活力试验来评估独立于蛋白质含量变化的活力.

主要成果:

DMEM/F-12和 gentamicin 的组合显示出最高水平的PCKS 可行性.
稳定的细胞活力在使用DMEM和 gentamicin时维持了长达10天.
总蛋白质含量在10天内显著下降,而LDH测定提供了对细胞膜损伤的见解.

结论:

优化化参数,特别是DMEM/F-12介质与甘他素,提高了PCKS对短期和长期研究的适用性.
对于毒理学和药理学研究来说,PCKS可以作为一个强大的模型.
进一步的研究可以利用这些优化的条件进行各种测试和调查.

关键词:

这就是为什么PCKS PCKS.我们的PCTS是PCTS.精密切割的脏切片,精确切割的脏切片.细胞活力细胞的活力.脏脏脏脏

更多相关视频

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

Published on: April 30, 2021

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

Optimized and Simplified Technique for the Production and Culture of Precision-Cut Liver Slices

Published on: November 22, 2024

相关概念视频

Reducing Line Loss

Reducing Line Loss

In a three-phase circuit, line loss is an indicator of energy dissipated as heat due to the resistance of transmission lines. To address this, incorporating transformers into the system—a step-up transformer at the source and a step-down transformer at the load—is a strategic solution. Two three-phase transformers are introduced to improve this.
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss in...

Methods of Medium Optimization

Methods of Medium Optimization

Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...