相关实验视频

Updated: Jun 20, 2025

Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases

Ultrasound-guided Intracardiac Injection of Human Mesenchymal Stem Cells to Increase Homing to the Intestine for Use in Murine Models of Experimental Inflammatory Bowel Diseases

Published on: September 1, 2017

在败血症模型中,11β氧类固醇脱酶1型转基因介质干细胞减轻炎症

Rahul Y Mahida¹, Zhengqiang Yuan², Krishna K Kolluri³

¹Birmingham Acute Care Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom.

Frontiers in bioengineering and biotechnology

|July 22, 2024

概括

转基因介质干细胞 (tMSCs) 过度表达HSD-1在败血症模型中减少了炎症. 这种增强的细胞疗法对治疗与败血症相关的炎症状况充满希望.

科学领域:

免疫学免疫学免疫学
再生医学是一种再生医学.
分子生物学分子生物学

背景情况:

介质干细胞 (MSC) 治疗在败血症模型中显示出抗炎潜力.
损伤的膜巨细胞 (AM) 11β-基类固醇脱酶-1型 (HSD-1) 信号传递有助于导致败血症的炎症.
过度表达HSD-1的转基因MSCs (tMSCs) 可能提供增强的抗炎作用.

研究的目的:

研究HSD-1过度表达tMSCs在减少炎症方面的疗效.
评估HSD-1转基因表达和MSC治疗的协同效应.

主要方法:

经过基因改造,MSCs使用了隐形病毒载体来过度表达HSD-1.
证实了HSD-1活动和MSC表型.
用巨细胞进行共同培养实验,并在小鼠败血症模型中进行体内研究 (切卡结合和穿刺).

主要成果:

感染过的MSC保持了其表型,并表现出功能性HSD-1活性.
HSD-1 tMSCs抑制了巨细胞从巨细胞中释放的促炎性细胞因子.
与对照tMSC相比,HSD-1tMSCs在小鼠败血症模型中显著减轻了中性恋炎症.

结论:

在MSC中HSD-1的协同表达在败血症模型中增强了抗炎作用.
在减少炎症方面,HSD-1tMSC疗法比单独的MSC疗法更有效.
需要对hsd-1tmsc在败血症相关的肺损伤和其他炎症性疾病中进行进一步的研究.

关键词:

在HSD-1中使用HSD-1.巨细胞是一个巨细胞.中介细胞干细胞中介细胞干细胞.这是一种血症.转化过程中的转化.

更多相关视频

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell MSC Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell MSC Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Published on: March 18, 2017

Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells

Author Spotlight: Advancing Treatment Approaches with Adipose-Derived Stem Cells

Published on: January 12, 2024

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...