用于组织工程应用的酸盐和聚乙烯醇制造的水凝的制备和表征
Ziyu Wang1, Nasif Mahmood1, Januka Budhathoki-Uprety1
1Wilson College of Textiles, North Carolina State University, Raleigh, North Carolina 27695, United States.
ACS applied bio materials
|July 22, 2024
概括
这项研究开发了用于生物医学用途的新型奇多-多 (?? 乙烯醇) 水凝. 高分子量奇多水凝显示出增强的细胞增殖和可调节性质,表明它们在组织工程应用中的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 聚合物化学 聚合物化学
- 组织工程是组织工程.
背景情况:
- 由于其高含水量和可调节性质,水凝对于生物医学应用至关重要.
- 奇托 (CS) 和聚乙醇 (PVA) 是生物相容的聚合物,有可能制造水凝.
- 化学交叉连接和冷干燥是创建稳定的水凝结构的常见方法.
研究的目的:
- 合成和鉴定不同分子量和度的基托多聚乙醇 (CS-PVA) 水凝.
- 评估物理化学性质,包括毛孔大小,水友性,胀和降解行为.
- 用人类皮肤纤维细胞来评估合成的水凝的细胞相容性和细胞活力.
主要方法:
- 用两种不同分子量的CS与PVA混合,与四乙烯正酸盐 (TEOS) 交联并冷干燥的CS制备CS-PVA水凝.
- 描述包括福里埃变换红外光谱 (FTIR),扫描电子显微镜 (SEM),拉伸强度,接触角度,胀和体外降解研究.
- 用人类皮肤纤维细胞的 alamarBlue 和活/死试验来评估细胞兼容性.
主要成果:
- SEM分析显示,基托桑度和分子量影响了水凝孔径大小.
- 增加基托桑含量减少了接触角度,表明增强的水友性.
- 试验室降解表现出与酸盐度/分子量之间非线性时间依赖的关系,受孔径大小的影响.
- 所有的CS-PVA水凝都表现出良好的细胞增殖,高分子量奇多样本表现特别好.
结论:
- 通过调整酸盐含量和分子量,可以成功制造具有可调节性质的CS-PVA水凝.
- 开发的水凝表现出有利的物理化学特性和出色的细胞兼容性,支持细胞增殖.
- 这些发现表明,CS-PVA水凝,特别是具有高分子量奇多的水凝,对包括组织工程在内的各种生物医学应用具有重大前景.
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