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Updated: Jun 20, 2025

Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells
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Author Spotlight: Optimizing Apoplast Protein Extraction for Efficient Recovery of Recombinant Proteins from Plant Cells

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基于芽细胞提取的方法来提高植物产生的重组蛋白质的纯度

Huiying Ji1, Tianhao Zhang2, Han Li3

  • 1Shandong Academy of Medical Sciences, Shandong First Medical University; State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences.

Journal of visualized experiments : JoVE
|July 22, 2024
PubMed
概括

这项研究提出了一种新的方法,用于从植物质中净化重组蛋白质,产生高度净化的治疗蛋白质. 这种方法克服了与植物性生产系统传统的全溶性蛋白质提取相关的挑战.

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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