相关实验视频

Updated: Jun 20, 2025

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Published on: March 16, 2022

化学性脂蛋白用于莱普托西罗斯疫苗:免疫性和保护潜力

Rafael Carracena de Souza Tapajóz¹, Francisco Denis Souza Santos¹, Natasha Rodrigues de Oliveira¹

¹Biotechnology Center, Technological Development Center, Federal University of Pelotas, Pelotas, RS, Brazil.

Applied microbiology and biotechnology

|July 22, 2024

概括

这项研究探讨了一种新型的化学脂蛋白疫苗候选人,用于治疗严重的动物传播疾病 - - 螺杆菌病. 虽然它成功刺激了IgG抗体,但它没有提供对毒性LeptoSpira挑战的保护.

科学领域:

兽医医学兽医医学兽医医学
免疫学免疫学免疫学
传染性疾病传染性疾病

背景情况:

莱普托斯皮罗斯是全球重要的被忽视的动物性传染病,患病率和死亡率很高.
疫苗接种是白螺旋病的关键预防策略.
外膜脂蛋白 (LIC12287,LIC11711,LIC13259) 是Leptospira spp.的一种外膜脂蛋白. 由于它们的表面位置和免疫性,它们是潜在的疫苗目标.

研究的目的:

开发和评估一种针对白病的化学蛋白候选疫苗.
在一个仓鼠模型中,评估基米蛋白的免疫性和保护效果.

主要方法:

在保存的LeptoSpira脂蛋白上识别和表征T细胞和B细胞表位.
构建,表达和净化一个嵌合蛋白疫苗.
用各种辅助剂或大肠杆菌菌素配制的奇美蛋白对仓鼠进行免疫.
挑战疫苗接种了毒性勒普托斯皮拉问询的仓鼠.

主要成果:

嵌合体蛋白被成功合成,表达和净化.
免疫接种诱导了显著的幽默性免疫反应,在接种后可以检测到IgG抗体.
在接种疫苗的仓鼠中,没有观察到对毒性勒普托斯皮拉的挑战有显著的保护.

结论:

嵌合体脂蛋白候选疫苗引发了强大的幽默性免疫反应.
需要进一步的研究来确定有效的白病疫苗的保护性抗原.

关键词:

莱普托斯皮拉 (Leptospira) 是一个类动物.辅助剂辅助剂辅助剂基米蛋白质是基米蛋白质中的一种.再组合细菌素是一种重组细菌.再组合无活化的大肠杆菌疫苗.复合子单位疫苗复合子单位疫苗

更多相关视频

Antigenic Liposomes for Generation of Disease-specific Antibodies

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

相关概念视频

Conjugated Proteins

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...

Cross-reactivity

Cross-reactivity