相关实验视频
Updated: Jun 20, 2025

09:20
Assessing Energy Substrate Oxidation In Vitro with 14CO2 Trapping
Published on: March 23, 2022
2.1K
二碳酸盐是一种关键调节剂,但不是光系统II中O2进化的基质
David J Vinyard1, Govindjee Govindjee2
1Department of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA. dvinyard@lsu.edu.
Photosynthesis research
|July 22, 2024
概括
光系统II (PSII) 使用光能分裂水并产生氧气. 尽管有证据,但一些理论建议二碳酸盐作为氧气来源,但实验表明在活性部位附近没有二碳酸盐.
科学领域:
- 生物化学 生物化学
- 光合作用 光合作用
- 结构生物学 结构生物学
背景情况:
- 光系统II (PSII) 对于光合作用至关重要,分解水以产生氧气 (O2) 并减少素.
- 虽然水是公认的O2来源,但涉及二碳酸盐的替代假设仍然存在.
- 了解O2进化机制对于理解光合作用至关重要.
研究的目的:
- 审查二碳酸盐在PSII活动和组装中的监管作用.
- 批判性地评估二碳酸盐是O2进化的基质的假设.
- 提出挑战基于二碳酸盐的O2进化理论的证据.
主要方法:
- 关于光系统II和碳酸盐的现有文献的综述.
- 分析生化,光谱和结构生物学数据.
- 对以氧为中心的二碳酸盐氧化过程的热力学评估.
主要成果:
- 二碳酸盐在PSII活动和组装中起着监管作用.
- 生物化学,光谱学和结构生物学实验没有在O2进化活性部位检测到二碳酸盐.
- 碳酸氧化存在热力学论证,但不证实它是基质.
结论:
- 现有证据支持水作为光合作用过程中唯一的O2来源.
- 碳酸作为O2进化的基质的假设没有得到实验数据的支持.
- 进一步的研究应该集中在光系统II中建立的水分裂机制上.
相关概念视频
The Calvin Benson Cycle
4.5K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.5K
Bicarbonate-Carbonic Acid Buffer
1.1K
The carbonic acid-bicarbonate buffer system is critical for maintaining the body's pH balance. It operates on the equilibrium:
1.1K
Photosystem II
70.1K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
70.1K
Photosystem I
62.0K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
62.0K
Oxygen Transport in the Blood
2.6K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
2.6K
The Calvin Cycle
73.8K
Overview
73.8K

